A novel in vivo mouse model for mechanically stimulated bone adaptation: a combined experimental and computational validation study

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Webster, Duncan J.¹; Morley, Philip L.¹; van Lenthe, G. Harry¹; Müller, Ralph¹

A novel in vivo mouse model for mechanically stimulated bone adaptation: a combined experimental and computational validation study

Comput Methods Biomech Biomed Eng. October 2008;11(5):435-441

©2008 Taylor & Francis

Affiliations

¹Institute for Biomechanics, ETH Zürich, Zürich, Switzerland
Abstract and keywords

To facilitate the investigation of bone formation, in vivo, in response to mechanical loading a caudal vertebra axial compression device (CVAD) has been developed to deliver precise mechanical loads to the fifth caudal vertebra (C5) of the C57BL/6 female mouse. A combined experimental and computational approach was used to quantify the micro-mechanical strain induced in trabecular and cortical components following static and dynamic loading using the CVAD. Cortical bone strains were recorded using micro-strain gages. Finite element (FE) models based on micro-computed tomography were constructed for all C5 vertebrae. Both theoretical and experimental cortical strains correlated extremely well (R²>0.96) for a Young's modulus of 14.8 GPa, thus validating the FE model. In this study, we have successfully applied mechanical loads to the C5 murine vertebrae, demonstrating the potential of this model to be used for in vivo loading studies aimed at stimulating both trabecular and cortical bone adaptation.

Keywords: trabecular bone; cortical bone; caudal vertebrae; mechanical loading; finite element analysis (FEA)
Links

DOI: 10.1080/10255840802078014

PubMed: 18612871

WoS: 000260457900002
History

Received: 2007-07-19

Revised: 2008-03-15

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Cited By (26)

Year	Entry
2012	Webster D, Wirth A, van Lenthe GH, Müller R. Experimental and finite element analysis of the mouse caudal vertebrae loading model: prediction of cortical and trabecular bone adaptation. Biomech Model Mechanobiol. January 2012;11(1-2):221-230.
2012	Christen P, van Rietbergen B, Lambers FM, Müller R, Ito K. Bone morphology allows estimation of loading history in a murine model of bone adaptation. Biomech Model Mechanobiol. March 2012;11(3-4):483-492.
2011	Schulte FA, Lambers FM, Kuhn G, Müller R. In vivo micro-computed tomography allows direct three-dimensional quantification of both bone formation and bone resorption parameters using time-lapsed imaging. Bone. March 2011;48(3):433-442.
2011	Lambers FM, Schulte FA, Kuhn G, Webster DJ, Müller R. Mouse tail vertebrae adapt to cyclic mechanical loading by increasing bone formation rate and decreasing bone resorption rate as shown by time-lapsed in vivo imaging of dynamic bone morphometry. Bone. December 2011;49(6):1340-1350.
2020	Ganesh T, Laughrey LE, Niroobakhsh M, Lara-Castillo N. Multiscale finite element modeling of mechanical strains and fluid flow in osteocyte lacunocanalicular system. Bone. August 2020;137:115328.
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