Microdamage, in the form of small cracks, may accumulate in trabecular bone loaded in fatigue. Specimens of bovine trabecular bone were loaded in compressive fatigue at one of four normalized stresses and loading was stopped after the specimens reached one of six maximum strains. Microdamage was identified using a fluorochrome staining technique, and microdamage parameters, including the number of damaged trabeculae and the damaged area fraction, were measured. No microdamage was observed during loading to strains below the yield strain;​ at higher strains, all microdamage parameters increased with increasing maximum compressive strain. Few significant differences were observed in the type or amount of microdamage accumulation between specimens loaded to the same maximum strain at different normalized stresses;​ however, more trabecular fractures were observed at high numbers of cycles, which corresponded to low normalized stresses