Heterogeneity of the mechanical properties of demineralized bone

Catanese, III, Joseph; Iverson, Erika P.; Ng, Ramford K.; Keaveny, Tony M.

Affiliations

¹Orthopaedic Biomechanics Laboratory, Department of Mechanical Engineering, University of California, Berkeley, CA 94720-1740, USA

²Joint Graduate Program in Bioengineering, University of California, Berkeley and San Francisco, CA, USA

³Department of Orthopaedic Surgery, University of California, San Francisco, CA, USA

Abstract and keywords

Knowledge of the mechanical properties of the collagenous component of bone is required for composite modeling of bone tissue and for understanding the age- and disease-related reductions in the ductility and strength of bone. The overall goal of this study was to investigate the heterogeneity of the mechanical properties of demineralized bone which remains unexplained and may be due to differences in the collagen structure or organization or in experimental protocols. Uniaxial tension tests were conducted to measure the elastic and failure properties of demineralized human femoral (n=10) and tibial (n=13) and bovine humeral (n=8) and tibial (n=8) cortical bone. Elastic modulus differed between groups (p=0.02), varying from 275±94 MPa (mean±SD) to 450±50 MPa. Similarly, ultimate stress varied across groups from 15±4.2 to 26±4.7 MPa (p=0.03). No significant differences in strain-to-failure were observed between any groups in this study (pooled mean of 8.4±1.6ffl; p=0.42). However, Bowman et al. (1996) reported an average ultimate strain of 12.3±0.5ffl for demineralized bovine humeral bone, nearly 40ffl higher than our value. Taken together, it follows that all the monotonic mechanical properties of demineralized bone can display substantial heterogeneity. Future studies directed at explaining such differences may therefore provide insight into aging and disease of bone tissue.

Keywords: Demineralized bone; Collagen; Mechanical properties; EDT

Links

DOI: 10.1016/S0021-9290(99)00128-1

PubMed: 10569717

WoS: 000083590200014

History

Accepted: 1999-5-29

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