The importance of murine cortical bone microstructure for microcrack initiation and propagation

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Voide, R.¹; Schneider, P.¹; Stauber, M.¹; van Lenthe, G. H.¹; Stampanoni, M.^2,3; Müller, R.¹

The importance of murine cortical bone microstructure for microcrack initiation and propagation

Bone. December 2011;49(6):1186-1193

©2011 Elsevier Inc. All rights reserved

Affiliations

¹Institute for Biomechanics, ETH Zurich, Zurich, Switzerland

²Swiss Light Source, Paul Scherrer Institut, Villigen, Switzerland

³Institute for Biomedical Engineering, University and ETH Zurich, Zurich, Switzerland
Abstract and keywords

In order to better understand bone postyield behavior and consequently bone failure behavior, this study aimed first to investigate cortical bone microstructure and second, to relate cortical bone microstructure to microdamage initiation and propagation in C57BL/6 (B6) and C3H/He (C3H) mice; two murine inbred strains known for their differences in bone phenotype. Murine femora of B6 and C3H were loaded axially under compression in a stepwise manner. For each loading step, 3D data sets at a nominal resolution of 700 nm were acquired by means of synchrotron radiation-based computed tomography. Cortical bone microstructure was divided into three phases: the canal network, the osteocyte lacunar system, and microdamage. Canal volume density and canal unit volume both correlated highly to crack number density (canal volume density: R² = 0.64, p < 0.005 and canal unit volume: R² = 0.75, p < 0.001). Moreover, the large canal units in C3H bone were responsible for more microdamage accumulation compared to B6 bones. This more pronounced microdamage accumulation due to large intracortical bone voids, which eventually leads to a fatal macrocrack (fracture), represents a potential contributing factor to the higher incidence of bone fractures in the elderly.

Keywords: Bone failure; Cortical bone microstructure; Microcracks; Synchrotron CT; Image-guided failure assessment
Links

DOI: 10.1016/j.bone.2011.08.011

PubMed: 21884836

WoS: 000297663500010
History

Received: 2011-01-17

Revised: 2011-08-04

Accepted: 2011-08-11

Online: 2011-08-22

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2014	Carriero A, Doube M, Vogt M, Busse B, Zustin J, Levchuk A, Schneider P, Müller R, Shefelbine SJ. Altered lacunar and vascular porosity in osteogenesis imperfecta mouse bone as revealed by synchrotron tomography contributes to bone fragility. Bone. April 2014;61:116-124.
2017	Rodriguez-Florez N, Carriero A, Shefelbine SJ. The use of XFEM to assess the influence of intra-cortical porosity on crack propagation. Comput Methods Biomech Biomed Eng. 2017;20(4):385-392.
2022	Buccino F, Bagherifard S, D'Amico L, Zagra L, Banfi G, Tromba G, Vergani LM. Assessing the intimate mechanobiological link between human bone micro-scale trabecular architecture and micro-damages. Eng Fract Mech. July 2022;270:108582.
2018	Levchuk A, Schneider P, Meier M, Vogel P, Donaldson F, Müller R. An automated step-wise micro-compression device for 3D dynamic image-guided failure assessment of bone tissue on a microstructural level using time-lapsed tomography. Front Mater. June 2018;5:32.
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