Accuracy of cancellous bone volume fraction measured by micro-CT scanning

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Ding, Ming¹; Odgaard, Anders¹; Hvid, Ivan¹

Accuracy of cancellous bone volume fraction measured by micro-CT scanning

J Biomech. 1999;32(3):323-326

Affiliations

¹Orthopaedic Research Laboratory, Aarhus University Hospital (ÅKH), Building 1A, Nørrebrogade 44, DK-8000 Aarhus C, Denmark
Abstract and keywords

Volume fraction, the single most important parameter in describing trabecular microstructure, can easily be calculated from three-dimensional reconstructions of micro-CT images. This study sought to quantify the accuracy of this measurement. One hundred and sixty human cancellous bone specimens which covered a large range of volume fraction (9.8–39.8%) were produced. The specimens were micro-CT scanned, and the volume fraction based on Archimedes’ principle was determined as a reference. After scanning, all micro-CT data were segmented using individual thresholds determined by the scanner supplied algorithm (method I). A significant deviation of volume fraction from method I was found; both the y-intercept and the slope of the regression line were significantly different from those of the Archimedes-based volume fraction (p<0.001). New individual thresholds were determined based on a calibration of volume fraction to the Archimedes-based volume fractions (method II). The mean thresholds of the two methods were applied to segment 20 randomly selected specimens. The results showed that volume fraction using the mean threshold of method I was underestimated by 4% (p=0.001), whereas the mean threshold of method II yielded accurate values. The precision of the measurement was excellent. Our data show that care must be taken when applying thresholds in generating 3-D data, and that a fixed threshold may be used to obtain reliable volume fraction data. This fixed threshold may be determined from the Archimedes-based volume fraction of a subgroup of specimens. The threshold may vary between different materials, and so it should be determined whenever a study series is performed.

Keywords: Volume fraction; Accuracy; Microtomography; Cancellous bone; Threshold
Links

DOI: 10.1016/S0021-9290(98)00176-6

PubMed: 10093033

WoS: 000079106300012
History

Accepted: 1998-10-25

Online: 1999-05-04

Cited Works (8)

Year	Entry
1990	Kuhn JL, Goldstein SA, Feldkamp LA, Goulet RW, Jesion G. Evaluation of a microcomputed tomography system to study trabecular bone structure. J Orthop Res. 1990;8(6):833-842.
1997	Ding M, Dalstra M, Danielsen CC, Kabel J, Hvid I, Linde F. Age variations in the properties of human tibial trabecular bone. J Bone Joint Surg. November 1997;79B(6):995-1002.
1990	Sharp DJ, Tanner KE, Bonfield W. Measurement of the density of trabecular bone. J Biomech. 1990;23(8):853-857.
1993	Odgaard A, Gundersen HJG. Quantification of connectivity in cancellous bone, with special emphasis on 3-D reconstructions. Bone. March–April 1993;14(2):173-182.
1996	Müller R, Koller B, Hildebrand T, Laib A, Gianolini S, Rüegsegger P. Resolution dependency of microstructural properties of cancellous bone based on three-dimensional μ-tomography. Technol Health Care. April 1996;4(1):113-119.
1997	Odgaard A. Three-dimensional methods for quantification of cancellous bone architecture. Bone. 1997;20(4):315-328.
1996	Rüegsegger P, Koller B, Müller R. A microtomographic system for the nondestructive evaluation of bone architecture. Calcif Tiss Int. 1996;58(1):24-29.
1989	Feldkamp LA, Goldstein SA, Parfitt MA, Jesion G, Kleerekoper M. The direct examination of three‐dimensional bone architecture in vitro by computed tomography. J Bone Miner Res. 1989;4(1):3-11.

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Year	Entry
2008	Zhang Y, He Z, Fan S, He K, Li C. Automatic thresholding of micro-CT trabecular bone images. Proceedings of the International Conference on BioMedical Engineering and Informatics (BMEI 2008); May 27-30, 2008; Sanya, Hainan, China.23-27.
2001	Fajardo RJ, Müller R. Three‐dimensional analysis of nonhuman primate trabecular architecture using micro‐computed tomography. Am J Phys Anthropol. 2001;115(4):327-336.
2000	Ding M, Hvid I. Quantification of age-related changes in the structure model type and trabecular thickness of human tibial cancellous bone. Bone. March 2000;26(3):291-295.
2000	Day JS, Ding M, Odgaard A, Sumner DR, Hvid I, Weinans H. Parallel plate model for trabecular bone exhibits volume fraction-dependent bias. Bone. November 2000;27(5):715-720.
2002	Hara T, Tanck E, Homminga J, Huiskes R. The influence of microcomputed tomography threshold variations on the assessment of structural and mechanical trabecular bone properties. Bone. July 2002;31(1):107-109.
2002	Hu JH, Ding M, Søballe K, Bechtold JE, Danielsen CC, Day JS, Hvid I. Effects of short-term alendronate treatment on the three-dimensional microstructural, physical, and mechanical properties of dog trabecular bone. Bone. November 2002;31(5):591-597.
2020	Ganesh T, Laughrey LE, Niroobakhsh M, Lara-Castillo N. Multiscale finite element modeling of mechanical strains and fluid flow in osteocyte lacunocanalicular system. Bone. August 2020;137:115328.
2020	Stadelmann MA, Schenk DE, Maquer G, Lenherr C, Buck FM, Bosshardt DD, Hoppe S, Theumann N, Alkalay RN, Zysset PK. Conventional finite element models estimate the strength of metastatic human vertebrae despite alterations of the bone's tissue and structure. Bone. December 2020;141:115598.
2003	Ding M, Day JS, Burr DB, Mashiba T, Hirano T, Weinans H, Sumner DR, Hvid I. Canine cancellous bone microarchitecture after one year of high-dose bisphosphonates. Calcif Tiss Int. June 2003;72(6):737-744.
2003	Giesen EBW, Ding M, Dalstra M, van Eijden TMGJ. Architectural measures of the cancellous bone of the mandibular condyle identified by principal components analysis. Calcif Tiss Int. 2003;73(3):225-231.
2004	Cooper DML, Matyas JR, Katzenberg MA, Hallgrimsson B. Comparison of microcomputed tomographic and microradiographic measurements of cortical bone porosity. Calcif Tiss Int. May 2004;75(5):437-447.
2008	Chevalier Y, Charlebois M, Pahr D, Varga P, Heini P, Schneider E, Zysset P. A patient-specific finite element methodology to predict damage accumulation in vertebral bodies under axial compression, sagittal flexion and combined loads. Comput Methods Biomech Biomed Eng. October 2008;11(5):477-487.
2007	Chevalier Y, Pahr D, Allmer H, Charlebois M, Zysset P. Validation of a voxel-based FE method for prediction of the uniaxial apparent modulus of human trabecular bone using macroscopic mechanical tests and nanoindentation. J Biomech. 2007;40(15):3333-3340.
2008	Perilli E, Baleani M, Öhman C, Fognani R, Baruffaldi F, Viceconti M. Dependence of mechanical compressive strength on local variations in microarchitecture in cancellous bone of proximal human femur. J Biomech. 2008;41(2):438-446.
2008	Renders GAP, Mulder L, Langenbach GEJ, van Ruijven LJ, van Eijden TMGJ. Biomechanical effect of mineral heterogeneity in trabecular bone. J Biomech. August 2008;41(13):2793-2798.
2005	Yeni YN, Christopherson GT, Dong XN, Kim D-G, Fyhrie DP. Effect of microcomputed tomography voxel size on the finite element model accuracy for human cancellous bone. J Biomech Eng. February 2005;127(1):1-8.
2015	Kaynia N, Soohoo E, Keaveny TM, Kazakia GJ. Effect of intraspecimen spatial variation in tissue mineral density on the apparent stiffness of trabecular bone. J Biomech Eng. January 2015;137(1):011010.
2002	Ding M, Odgaard A, Danielsen CC, Hvid I. Mutual associations among microstructural, physical and mechanical properties of human cancellous bone. J Bone Joint Surg. August 2002;84B(6):900-907.
2004	Waarsing JH, Day JS, Weinans H. An improved segmentation method for in vivo μCT imaging. J Bone Miner Res. 2004;19(10):1640-1650.
2007	Perilli E, Baruffaldi F, Visentin M, Bordini B, Traina F, Cappello A, Viceconti M. MicroCT examination of human bone specimens: effects of polymethylmethacrylate embedding on structural parameters. J Microsc (Oxford). 2007;225(pt 2):192-200.
2001	Day JS, Ding M, van der Linden JC, Hvid I, Sumner DR, Weinans H. A decreased subchondral trabecular bone tissue elastic modulus is associated with pre‐arthritic cartilage damage. J Orthop Res. September 2001;19(5):914-918.
2002	Ding M, Odgaard A, Linde F, Hvid I. Age-related variations in the microstructure of human tibial cancellous bone. J Orthop Res. May 2002;20(3):615-621.
2004	Day JS, Ding M, Bednarz P, van der Linden JC, Mashiba T, Hirano T, Johnston CC, Burr DB, Hvid I, Sumner DR, Weinans H. Bisphosphonate treatment affects trabecular bone apparent modulus through micro-architecture rather than matrix properties. J Orthop Res. May 2004;22(3):465-471.
2021	Ding M, Overgaard S. 3-D microarchitectural properties and rod- and plate-like trabecular morphometric properties of femur head cancellous bones in patients with rheumatoid arthritis, osteoarthritis, and osteoporosis. J Orthop Translat. May 2021;28:159-168.
2008	Kazakia GJ, Burghardt AJ, Cheung S, Majumdar S. Assessment of bone tissue mineralization by conventional x-ray microcomputed tomography: comparison with synchrotron radiation microcomputed tomography and ash measurements. Med Phys. 2008;35(7):3170-3179.
2010	Ramsey DS. Effects of Gamma Irradiation on the Damage Processes in Human Trabecular Bone [Master's thesis]. Cleveland, OH: Case Western Reserve University; October 2010.
2003	Kim CH. In Vivo Trabecular Bone Response to Mechanical Loading and Parathyroid Hormone Stimulation [PhD thesis]. Columbia University; 2003.
2006	Fritton JC. Mechanical Loading Induced Adaptation of the Mouse Tibia [PhD thesis]. Ithaca, NY: Cornell University; January 2006.
2015	Rowland CR. Development of Cartilage-Derived Matrix Scaffolds Via Crosslinking Decellularization, and Ice-templating [PhD thesis]. Duke University; 2015.
2005	Day JS. Bone Quality: The Mechanical Effects of Microarchitecture and Matrix Properties [PhD thesis]. Erasmus University Rotterdam; 2005.
2002	Follet H. Caractérisation Biomécanique Et Modélisation 3D Par Imagerie X Et IRM Haute Résolution De L’os Spongieux Humain: Evaluation Du Risque Fracturaire [PhD thesis]. Institut national des sciences appliquées de Lyon (INSA Lyon); 2002.
2009	Moesen M. Modeling of the Geometry and Mechanical Behavior of Bone Scaffolds [PhD thesis]. Leuven, Belgium: Katholieke Universiteit Leuven; June 2009.
2006	Yang S. Animal Experiment (rabbit) to Demonstrate Changes in Trabecular Bone Mechanical Properties Over Time Using Finite Element Analysis [PhD thesis]. Louisville, KY: University of Louisville; December 2006.
2012	Tozzi G. In Vitro Studies of Bone-Cement Interface and Related Work on Cemented Acetabular Replacement [PhD thesis]. Portsmouth, England: University of Portsmouth; May 29, 2012.
2009	Bower AL. An Investigation of Trabecular Bone Morphology [PhD thesis]. State College, PA: Pennsylvania State University; December 2009.
2004	De Ranieri A. Morphologic, Mechanical, and Molecular Aspects of TGF-Β2 Enhanced Implant Fixation [PhD thesis]. Rush University; 2004.
2010	Liu S. Development and Initial Testing of a Rat Model for Particle-Induced Impaired Implant Fixation [PhD thesis]. Rush University; 2010.
2004	Mittra ES. Assessment of Trabecular Bone Quality Using Microstructure, Micro-Mechanics and Micro-Finite Element Modeling [PhD thesis]. Stony Brook, NY: Stony Brook University; May 2004.
2012	Dall'Ara E. QCT Based Finite Element Models of the Human Vertebra and Femur: Validation With Experiments and Comparison With Bone Densitometry [PhD thesis]. Vienna University of Technology; October 2012.
2003	Pardy CK. The Effects of Doxycycline on the Mechanical and Morphometric Properties of Early and Established Osteoarthritic Bone in a Canine Model Following Anterior Cruciate Ligament Injury [Master's thesis]. Calgary, AB: University of Calgary; October 2003.
2005	Cooper DML. 3D Micro-CT Imaging of Human Cortical Bone Porosity: A Novel Method for Estimating Age At Death [PhD thesis]. Calgary, AB: University of Calgary; December 2005.
2014	Nawathe S. Micromechanics of the Human Proximal Femur: Role of Microstructure and Tissue-Level Ductility on Femoral Strength [PhD thesis]. Berkeley, CA: Berkeley, University of California; 2014.
2013	Soicher MA. Fabrication and Characterization of Remineralized Bone Matrices (RBM) for Use as Bone Tissue Engineering Substrates [PhD thesis]. Davis, University of California; 2013.