Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering

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Vunjak-Novakovic, Gordana¹; Obradovic, Bojana¹; Martin, Ivan¹; Bursac, Predrag M.¹; Langer, Robert¹; Freed, Lisa E.¹

Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering

Biotechnol Prog. March–April 1998;14(2):193-202

©1998 American Chemical Society and American Institute of Chemical Engineers

Affiliations

¹Division of Health Sciences and Technology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139
Abstract

Cell seeding of three-dimensional polymer scaffolds is the first step of the cultivation of engineered tissues in bioreactors. Seeding requirements of large scaffolds to make implants for potential clinical use include: (a) high yield, to maximize the utilization of donor cells, (b) high kinetic rate, to minimize the time in suspension for anchorage-dependent and shear-sensitive cells, and (c) high and spatially uniform distribution of attached cells, for rapid and uniform tissue regeneration. Highly porous, fibrous polyglycolic acid scaffolds, 5–10 mm in diameter and 2–5 mm thick, were seeded with bovine articular chondrocytes in well-mixed spinner flasks. Essentially, all cells attached throughout the scaffold volume within 1 day. Mixing promoted the formation of 20–32-μm diameter cell aggregates that enhanced the kinetics of cell attachment without compromising the uniformity of cell distribution. The kinetics and possible mechanisms of cell seeding were related to the formation of cell aggregates by a simple mathematical model that can be used to optimize seeding conditions for cartilage tissue engineering.
Links

DOI: 10.1021/bp970120j

PubMed: 9548769

WoS: 000073011600003
History

Accepted: 1997-12-01

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Cited By (39)

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