Cartilage production by rabbit articular chondrocytes on polyglycolic acid scaffolds in a closed bioreactor system

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Dunkelman, Noushin S.¹; Zimber, Michael P.¹; LeBaron, Richard G.¹; Pavelec, Rebecca¹; Kwan, Michael¹; Purchio, A. F.¹

Cartilage production by rabbit articular chondrocytes on polyglycolic acid scaffolds in a closed bioreactor system

Biotechnol Bioeng. May 20, 1995;46(4):299-305

©1995 John Wiley & Sons, Inc.

Affiliations

¹Advanced Tissue Scinces, 10933 North Torrey Pines Road, La Jolla, California 92037-1005
Abstract and keywords

Rabbit articular chondrocytes were seeded onto three-dimensional polyglycolic acid (PGA) scaffolds and placed into a closed bioreactor system. After 4 weeks of growth, meshes were examined for cartilage formation. Gross examination revealed solid, glistening material that had the appearance of cartilaginous tissue. Histologic examination revealed cell growth and deposition of extracellular matrix throughout the mesh with a less dense central core. Alcian blue and Safranin 0 staining showed deposition of glycosaminoglycans (GAGs). Immunostaining showed positive reactivity for type II collagen and chondroitin sulfate and no reactivity for type I collagen. Biochemical analysis showed collagen and GAG values to be 15% and 25% dry weight, respectively. Our results indicate that this type of system compares well with those previously described and should be useful for producing cartilage for evaluation in a clinical setting.

Keywords: cartilage; polyglycolic acid; chondrocytes
Links

DOI: 10.1002/bit.260460402

PubMed: 18623316

WoS: A1995QU43400001
History

Received: 1994-08-03

Accepted: 1994-12-21

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Cited By (29)

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2002	Mauck RL, Seyhan SL, Ateshian GA, Hung CT. Influence of seeding density and dynamic deformational loading on the developing structure/function relationships of chondrocyte-seeded agarose hydrogels. Ann Biomed Eng. September 2002;30(8):1046-1056.
2004	Hung CT, Mauck RL, Wang CC-B, Lima EG, Ateshian GA. A paradigm for functional tissue engineering of articular cartilage via applied physiologic deformational loading. Ann Biomed Eng. January 2004;32(1):35-49.
2000	Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials. December 15, 2000;21(24):2529-2543.
1999	Carver SE, Heath CA. Increasing extracellular matrix production in regenerating cartilage with intermittent physiological pressure. Biotechnol Bioeng. 1999;62(2):166-174.
1998	Vunjak-Novakovic G, Obradovic B, Martin I, Bursac PM, Langer R, Freed LE. Dynamic cell seeding of polymer scaffolds for cartilage tissue engineering. Biotechnol Prog. March–April 1998;14(2):193-202.
2000	Hung CT, Ross Henshaw D, Wang CC-B, Mauck RL, Raia F, Palmer G, Grace Chao P-H, Mow VC, Ratcliffe A, Valhmu WB. Mitogen-activated protein kinase signaling in bovine articular chondrocytes in response to fluid flow does not require calcium mobilization. J Biomech. January 2000;33(1):73-80.
2000	Mauck RL, Soltz MA, Wang CCB, Wong DD, Chao P-HG, Valhmu WB, Hung CT, Ateshian GA. Functional tissue engineering of articular cartilage through dynamic loading of chondrocyte-seeded agarose gels. J Biomech Eng. June 2000;122(3):252-260.
2003	Masuda K, Sah RL, Hejna MJ, Thonar EJ-MA. A novel two-step method for the formation of tissue-engineered cartilage by mature bovine chondrocytes: the alginate-recovered-chondrocyte (ARC) method. J Orthop Res. January 2003;21(1):139-148.
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