Low Frequency Stimulation of Stem Cells in Dynamic Culture Modulates Differentiation Pathways

Links

URL: https://escholarship.mcgill.ca/concern/theses/zs25xc98r

Abstract (English)

Living cells, depending on their physiological functions, are subjected to a variety of mechanical stimulation. The magnitude and frequency of such mechanical stimulation varies dramatically in different organs. Oscillatory mechanical stimulation at relatively high frequancies, as occurs in walking, respiration and circulation, is one of the most extensively studied schemes. However, the stimulation at extremely low frequencies is rarely examined. This research investigates the effects of relatively low frequency mechanical stimulation in molecular scale, on different cell types. Throughout the work presented in this document, the emphasis was on the stem cells differentiation, and primary cells dedifferentiation. The results suggested that performing extremely slow activities, namely low frequency movements, significantly affects the differentiation pathways of stem cells. In addition, it was found that slow movement of surface culture area enhances phenotypical characteristics of primary cells.

Abstract (French)

Toutes les cellules vivantes, selon leur fonctions physiologiques, sont soumises à différentes stimulations mécaniques. L'ampleur et la fréquence de ces stimulations mécaniques varies considérablement d'un organe à un autre. Les stimulations oscillantes dues notamment à la marche, la respiration et la circulation sanguine sont largement étudiées. Par contre, les travaux concernant les stimulations a très faibles fréquences sont rare. Cette recherche examine les effets sur différents types de molécules, des stimulations mécaniques à relativement basse fréquence, à l'échelle moléculaire. Tout au long du travail présenté ici, l'accent a été mis sur la différenciation des cellules souches et la dedifférenciation des cellules primaires. Les résultats suggèrent que la pratique d'activités extrêmement lentes, à savoir les mouvements à basse fréquence, affectent, de manière significative, le mécanisme de différentiation des cellules souches. En outre, il a été constaté que les mouvements lents à la surface des cultures améliorent les caractéristiques phénotypiques des cellules primaires.

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