Techniques for mechanical stimulation of cells in vitro:​ a review

Brown, Thomas D.<sup>1</sup>

Affiliations

¹Orthopaedic Biomechanics Laboratory, Department of Orthopaedic Surgery, University of Iowa, 2181 Westlawn Bldg, Iowa City, IA 52242, USA

Abstract

A wide variety of laboratory apparatuses have been devised for mechanical stimulation of cell and tissue cultures. This article reviews the functional attributes of several dozen systems developed for that purpose, including their major advantages and disadvantages. These devices can be categorized in terms of their primary loading modality: compression (hydrostatic pressure or direct platen contact), longitudinal stretch, bending, axisymmetric substrate bulge, in-plane substrate distention, fluid shear stress, or combined substrate distention and fluid shear.

Links

DOI: 10.1016/S0021-9290(99)00177-3

PubMed: 3379508

WoS: A1988N883700010

History

Revised: 2000-01-1

Online: 2000-01-10

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2003	Simmons CA, Matlis S, Thornton AJ, Chen S, Wang C-Y, Mooney DJ. Cyclic strain enhances matrix mineralization by adult human mesenchymal stem cells via the extracellular signal-regulated kinase (ERK1/2) signaling pathway. J Biomech. August 2003;36(8):1087-1096.
2008	Bacabac RG, Mizuno D, Schmidt CF, MacKintosh FC, Van Loon JJWA, Klein-Nulend J, Smit TH. Round versus flat: bone cell morphology, elasticity, and mechanosensing. J Biomech. 2008;41(7):1590-1598.
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2003	Darling EM, Athanasiou KA. Articular cartilage bioreactors and bioprocesses. Tissue Eng. February 2003;9(1):9-26.
2015	Scully N. Differentiation of Osteoblasts to Osteocytes in 3D Type I Collagen Gels: A Novel Tool to Study Osteocyte Responses to Mechanical Loading [PhD thesis]. Cardiff, UK: Cardiff University; April 2015.
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2005	Androjna C. Natural Extracellular Matrices for Tendon Tissue Engineering [PhD thesis]. Cleveland State University; May 2005.
2016	Miao J. An Improved in Vitro Cell-Shearing Device for Applying High Shear Stress Impulse on Cells [Master's thesis]. Drexel University; March 2016.
2013	Dirks RC. The Individual and Combined Effects of Exercise and Collagenase on the Rodent Achilles Tendon [PhD thesis]. Indianapolis, IN: Indiana University; October 2013.
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2013	Shafieyan Y. Mechanical Stimulation of Orthopaedic Tissues: Regulation of Bone Resorption and Solute Transport Through Articular Cartilage [PhD thesis]. McGill University; August 2013.
2001	Voldman J. A Microfabricated Dielectrophoretic Trapping Array for Cell-Based Biological Assays [PhD thesis]. Cambridge, MA: Massachusetts Institute of Technology; January 2001.
2007	Lavagnino M. The Mechanotransduction Response of Tendon Cells to Tensile Loading [PhD thesis]. East Lansing, MI: Michigan State University; 2007.
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2008	Stevenson MP. Development of a Mechanical Cell Stimulation System [Master's thesis]. Queen's University; August 2008.
2006	Hannay G. Mechanical and Electrical Environments to Stimulate Bone Cell Development [PhD thesis]. Queensland University of Technology; August 2006.
2004	Darling EM. Phenotype of Passaged, Zonal Articular Chondrocytes [PhD thesis]. Houston, TX: Rice University; July 2004.
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2015	Tseng L-F. Shape Memory Electrospun Scaffolds for Cell Mechanobiology and Bone Regenerative Applications [PhD thesis]. University of Syracuse; December 2015.
2005	McGarry JG. Computational and Experimental Investigation of Bone Cell Response to Mechanical Stimuli [PhD thesis]. Trinity College Dublin; September 2005.
2003	Ominsky MS. Effects of Hydrostatic Pressure, Biaxial Strain, and Fluid Shear on Osteoblastic Cells: Mechanotransduction Via NF-ΚB, MAP Kinase, and AP-1 Pathways [PhD thesis]. University of Michigan; 2003.
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