Area and strain measurement pose special problems in experiments designed to characterize the constitutive response of ligamentous tissue. Contact area measurements tools yield inaccurate results because the specimen is easily deformed. In this study, a 3D non-contact optical digitizer that relies on the principle of structured lighting is used to develop geometric models of all the ligaments of the knee joint, from which accurate calculations of cross-sectional area can be made. Similarly, it is important that the strain measurement technique used does not disturb the strain state of the specimen. Furthermore, full-field strain measurements are necessary because ligamentous tissue is inhomogeneous and local strain measurements can be misleading. Thus, this study demonstrates the application of a full-field 3D non-contact strain measurement system that relies on principles of photogrammetry and pattern recognition to compute the surface strain distribution on the Medial Collateral Ligament (MCL) of the human knee joint.