Elastic anisotropy of uniaxial mineralized collagen fibers measured using two-directional indentation:​ effects of hydration state and indentation depth

Spiesz, Ewa M.<sup>1</sup>; Roschger, Paul<sup>2</sup>; Zysset, Philippe K.<sup>3</sup>

Affiliations

¹Institute of Lightweight Design and Structural Biomechanics, Vienna University of Technology, Gusshausstrasse 27-29, A-1040 Vienna, Austria

²Ludwig-Boltzmann Institute of Osteology and Fourth Medical Department, Hanusch Hospital and UKH Meidling Kundratstrasse 37, A-1120 Vienna, Austria

³Institute for Surgical Technology & Biomechanics, University of Bern, Stauffacherstrasse 78, CH-3014 Bern, Switzerland

Abstract and keywords

Mineralized turkey leg tendon (MTLT) is an attractive model of mineralized collagen fibers, which are also present in bone. Its longitudinal structure is advantageous for the relative simplicity in modeling, yet its anisotropic elastic properties remain unknown. The aim of this study was to quantify the extent of elastic anisotropy of mineralized collagen fibers by using nano- and microindentation to probe a number on MTLT samples in two orthogonal directions. The large dataset allowed to quantify of the extent of anisotropy, depending on the final indentation depth and on the hydration state of the sample.

Anisotropy was observed to increase with the sample re-hydration process. Artifacts of indentation in a transverse direction to the main axis of the mineralized tendons in re-hydrated condition were observed. The indentation size effect, that is, the increase of the measured elastic properties with decreasing sampling volume, reported previously on variety of materials, was also observed in MTLT. Indentation work was quantified for both directions of indentation in dried and re-hydrated conditions.

As hypothesized, MTLT showed a higher extent of anisotropy compared to cortical and trabecular bone, presumably due to the alignment of mineralized collagen fibers in this tissue.

Keywords: Mineralized turkey leg tendon (MTLT); Uniaxial mineralized collagen fibers; Anisotropy; Microindentation; Nanoindentation; Re-hydration; Indentation modulus; Indentation work

Links

DOI: 10.1016/j.jmbbm.2012.03.004

PubMed: 22664658

PubMedCentral: PMC3424420

WoS: 000308626300003

History

Received: 2011-08-2

Revised: 2012-03-6

Accepted: 2012-03-8

Online: 2012-03-20

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Cited By (12)

Year	Entry
2016	Mirzaali MJ, Schwiedrzik JJ, Thaiwichai S, Best JP, Michler J, Zysset PK, Wolfram U. Mechanical properties of cortical bone and their relationships with age, gender, composition and microindentation properties in the elderly. Bone. December 2016;93:196-211.
2015	Granke M, Does MD, Nyman JS. The role of water compartments in the material properties of cortical bone. Calcif Tiss Int. September 2015;97(3):292-307.
2020	Zhou J, Cui Z, Sevostianov I. Effect of saturation on the elastic properties and anisotropy of cortical bone. Int J Eng Sci. October 2020;155:103362.
2014	Faingold A, Cohen SR, Shahar R, Weiner S, Rapoport L, Wagner HD. The effect of hydration on mechanical anisotropy, topography and fibril organization of the osteonal lamellae. J Biomech. January 22, 2014;47(2):367-372.
2014	Achrai B, Bar-On B, Wagner D. Bending mechanics of the red-eared slider turtle carapace. J Mech Behav Biomed Mater. February 2014;30:223-233.
2020	Remache D, Semaan M, Rossi JM, Pithioux M, Milan J. Application of the Johnson-Cook plasticity model in the finite element simulations of the nanoindentation of the cortical bone. J Mech Behav Biomed Mater. January 2020;101:103426.
2015	Chittenden M, Najafi AR, Li J, Jasiuk I. Nanoindentation and ash content study of age dependent changes in porcine cortical bone. J Mech Med Biol. October 2015;15(5):1550074.
2022	Peruzzi CRP. Nanoscale Deformation Mechanisms and Yield Prediction of Lamellar Bone [PhD thesis]. Swiss Federal Institute of Technology Zürich; 2022.
2019	Groetsch A. Micro- and Nanomechanics of Mineralised Collagen Fibre Elasto-Plasticity [PhD thesis]. Ebinburgh, Scotland: Heriot-Watt University; November 2019.
2018	Wei J. Methods to Improve Indentation Results on Soft Materials and Anisotropic Biological Tissues [PhD thesis]. University of Illinois at Urbana-Champaign; 2018.
2014	Schwiedrzik JJ. Experimental, Theoretical and Numerical Investigation of the Nonlinear Micromechanical Properties of Bone [PhD thesis]. Universität Bern; 2014.
2013	Faingold A. Bone Elementary Units: Mechanical Properties and Structure [PhD thesis]. Weizmann Institute of Science; February 2013.