Mineralization of collagen may occur on fibril surfaces:​ evidence from conventional and high-voltage electron microscopy and three-dimensional imaging

Landis, William J.; Hodgens, Karen J.; Song, Min Ja; Arena, James; Kiyonaga, Sumiko; Marko, Michael; Owen, Cameron; McEwen, Bruce F.

Affiliations

¹Department of Orthopedic Surgery, Harvard Medical School and Children’s Hospital, Boston, Massachusetts 02115

²Wadsworth Center for Laboratories and Research, New York State Department of Health, Albany, New York 12201

Abstract

The interaction between collagen and mineral crystals in the normally calcifying leg tendons from the domestic turkey, Meleagris gallopavo,has been investigated at an ultrastructural level with conventional and high-voltage electron microscopy, computed tomography, and three-dimensional image reconstruction methods. Specimens treated by either aqueous or anhydrous techniques and resin-embedded were appropriately sectioned and regions of early tendon mineralization were photographed. On the basis of individual photomicrographs, stereoscopic pairs of images, and tomographic three-dimensional image reconstructions, platelet-shaped crystals may be demonstrated for the first time in association with the surface of collagen fibrils. Mineral is also observed in closely parallel arrays within collagen hole and overlap zones. The mineral deposition at these spatially distinct locations in the tendon provides insight into possible means by which calcification is mediated by collagen as a fundamental event in skeletal and dental formation among vertebrates.

Links

DOI: 10.1006/jsbi.1996.0066

PubMed: 8776885

WoS: A1996VD22500003

History

Received: 1996-01-23

Revised: 1996-04-15

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