Frequency distributions of tetracycline-based measurements: implications for the interpretation of bone formation indices in the absence of double-labeled surfaces

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Foldes, Joseph¹; Shih, Mei-Shu¹; Parfitt, A. Michael¹

Frequency distributions of tetracycline-based measurements: implications for the interpretation of bone formation indices in the absence of double-labeled surfaces

J Bone Miner Res. October 1990;5(10):1063-1067

©2023 American Society for Bone and Mineral Research

Affiliations

¹Department of Medicine and Bone and Joint Center, Henry Ford Hospital, Detroit, MI 48202
Abstract

The frequency distributions of mineral apposition rate (MAR) and mineralizing surface (MS), measured separately on the intracortical, endocortical, and cancellous surfaces in 46 normal subjects and 79 patients with postmenopausal osteoporosis, indicated that MAR has a finite lower limit of 0.3 μ/day (uncorrected for section obliquity) but that MS has no finite lower limit. We conclude that in the absence of labels MAR, and indices derived from it, must be treated as missing values, but that MS and indices with MS in the numerator should be allowed to take values of zero. To avoid infinite values for indices with MS in the denominator, we propose that osteoid mineralization rate (the reciprocal of mineralization lag time) and osteoblast vigor (the reciprocal of formation period) be used instead. For surfaces with genuine single labels (SL) but no double labels, we propose that MS is calculated as SL/2 and that for MAR either the lower limit of 0.3 or the mean measured value from other surfaces be used for calculating derived indices.
Links

DOI: 10.1002/jbmr.5650051010

PubMed: 2080717

WoS: A1990ED86500009
History

Received: 1990-01-05

Revised: 1990-05-14

Accepted: 1990-06-01

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2013	Willie BM, Birkhold AI, Razi H, Thiele T, Aido M, Kruck B, Schill A, Checa S, Main RP, Duda GN. Diminished response to in vivo mechanical loading in trabecular and not cortical bone in adulthood of female C57Bl/6 mice coincides with a reduction in deformation to load. Bone. August 2013;55(2):335-346.
2020	Novel-Catin E, Pelletier S, Fouque D, Roux J-P, Chapurlat R, D'Haese P, Behets G, Evenepoel P, Nickolas TL, Lafage-Proust M-H. Quantitative histomorphometric analysis of halved iliac crest bone biopsies yield comparable ROD diagnosis as full 7.5mm wide samples. Bone. September 2020;138:115460.
2020	Albiol L, Büttner A, Pflanz D, Mikolajewicz N, Birkhold AI, Kramer I, Kneissel M, Duda GN, Checa S, Willie BM. Effects of long-term sclerostin deficiency on trabecular bone mass and adaption to limb loading differ in male and female mice. Calcif Tiss Int. April 2020;106(4):415-430.
2020	Qiu S, Divine G, Warner E, Rao SD. Reference intervals for bone histomorphometric measurements based on data from healthy premenopausal women. Calcif Tiss Int. December 2020;107(6):543-550.
1992	Balena R, Shih M-S, Parfitt AM. Bone resorption and formation on the periosteal envelope of the ilium: a histomorphometric study in healthy women. J Bone Miner Res. December 1992;7(12):1475-1482.
1997	Han Z-H, Palnitkar S, Rao DS, Nelson D, Parfitt AM. Effects of ethnicity and age or menopause on the remodeling and turnover of iliac bone: implications for mechanisms of bone loss. J Bone Miner Res. April 1997;12(4):498-508.
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2010	Brodt MD, Silva MJ. Aged mice have enhanced endocortical response and normal periosteal response compared with young-adult mice following 1 week of axial tibial compression. J Bone Miner Res. September 2010;25(9):2006-2015.
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2013	Dempster DW, Compston JE, Drezner MK, Glorieux FH, Kanis JA, Malluche H, Meunier PJ, Ott SM, Recker RR, Parfitt AM. Standardized nomenclature, symbols, and units for bone histomorphometry: a 2012 update of the report of the ASBMR Histomorphometry Nomenclature Committee. J Bone Miner Res. January 2013;28(1):2-17.
2000	Bone HG, Greenspan SL, McKeever C, Bell N, Davidson M, Downs RW, Emkey R, Meunier PJ, Miller SS, Mulloy AL, Recker RR, Weiss SR, Heyden N, Musliner T, Suryawanshi S, Yates AJ, Lombardi A; The Alendronate / Estrogen Study Group. Alendronate and estrogen effects in postmenopausal women with low bone mineral density. J Clin Endocrinol Metab. February 2000;85(2):720-726.
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2004	Castillo AB. The Female Ferret (Mustela Putorius Furo) as an in Vivo Model for Skeletal Research [PhD thesis]. Davis, University of California; 2004.
2012	Tjhia CKH. Bone Quality of Patients With Atypical Fractures and Severely Suppressed Bone Turnover [PhD thesis]. Davis, University of California; 2012.