Compression tests have often been performed to assess the biomechanical properties of full-thickness articular cartilage. We tested whether the apparent homogeneous strain-dependent properties, deduced from such tests, reflect both strain- and depth-dependent material properties. Full-thickness bovine articular cartilage was tested by oscillatory confined compression superimposed on a static offset up to 45%, and the data fit to estimate modulus, permeability, and electrokinetic coefficient assuming homogeneity. Additional tests on partial-thickness cartilage were then performed to assess depth- and strain-dependent properties in an inhomogeneous model, assuming three discrete layers (i=1 starting from the articular surface, to i=3 up to the subchondral bone). Estimates of the zero-strain equilibrium confined compression modulus (H_A0), the zero-strain permeability (k_p0) and deformation dependence constant (M), and the deformation-dependent electrokinetic coefficient (k_e) differed among individual layers of cartilage and full-thickness cartilage. H_A0ⁱ increased from layer 1 to 3 (0.27 to 0.71 MPa), and bracketed the apparent homogeneous value (0.47 MPa). k_p0ⁱ decreased from layer 1 to 3 (4.6×10⁻¹⁵ to 0.50×10⁻¹⁵ m²/Pa s) and was less than the homogeneous value (7.3×10⁻¹⁵ m²/Pa s), while Mi increased from layer 1 to 3 (5.5 to 7.4) and became similar to the homogeneous value (8.4). The amplitude of kei increased markedly with compressive strain, as did the homogeneous value;​ at low strain, it was lowest near the articular surface and increased to a peak in the middle-deep region. These results help to interpret the biomechanical assessment of full-thickness articular cartilage.