The effect of cytoskeletal disruption on pulsatile fluid flow-induced nitric oxide and prostaglandin e2 release in osteocytes and osteoblasts

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McGarry, James G.^1,2; Klein-Nulend, Jenneke²; Prendergast, Patrick J.¹

The effect of cytoskeletal disruption on pulsatile fluid flow-induced nitric oxide and prostaglandin e2 release in osteocytes and osteoblasts

Biochem Biophys Res Commun. April 29, 2005;330(1):341-348

©2005 Elsevier Inc. All rights reserved.

Affiliations

¹Centre for Bioengineering, Department of Mechanical Engineering, Trinity College, Dublin, Ireland

²Department of Oral Cell Biology, ACTA—Universiteit van Amsterdam and Vrije Universiteit, Amsterdam, The Netherlands
Abstract and keywords

Fluid flowing through the bone porosity might be a primary stimulus for functional adaptation of bone. Osteoblasts, and osteocytes in particular, respond to fluid flow in vitro with enhanced nitric oxide (NO) and prostaglandin E₂ (PGE₂) release; both of these signaling molecules mediate mechanically-induced bone formation. Because the cell cytoskeleton is involved in signal transduction, we hypothesized that the pulsatile fluid flow-induced release of NO and PGE₂ in both osteoblastic and osteocytic cells involves the actin and microtubule cytoskeleton. In testing this hypothesis we found that fluid flow-induced NO response in osteoblasts was accompanied by parallel alignment of stress fibers, whereas PGE₂ response was related to fluid flow stimulation of focal adhesions formed after cytoskeletal disruption. Fluid flow-induced PGE₂ response in osteocytes was inhibited by cytoskeletal disruption, whereas in osteoblasts it was enhanced. These opposite PGE₂ responses are likely related to differences in cytoskeletal composition (osteocyte structure was more dependent on actin), but may occur via cytoskeletal modulation of shear/stretch-sensitive ion channels that are known to be dominant in osteocyte (and not osteoblast) response to mechanical loading.

Keywords: Osteocytic; Osteoblastic; Pulsatile fluid flow; Nitric oxide; Prostaglandin E₂; Cytoskeleton; Actin; Microtubules
Links

DOI: 10.1016/j.bbrc.2005.02.175

PubMed: 15781270

WoS: 000228253700048
History

Received: 2005-02-6

Revised: 2005-04-29

Online: 2005-03-10

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