Dependence of mechanical properties of trabecular bone on plate-rod microstructure determined by individual trabecula segmentation (ITS)

Zhou, Bin<sup>1</sup>; Liu, X. Sherry<sup>1,2</sup>; Wang, Ji<sup>1</sup>; Lu, X. Lucas<sup>1,3</sup>; Fields, Aaron J.<sup>4,5</sup>; Guo, X. Edward<sup>1</sup>

Affiliations

¹Bone Bioengineering Laboratory, Department of Biomedical Engineering, Columbia University, New York, New York, U.S.A.

²McKay Orthopaedic Research Laboratory, University of Pennsylvania, Philadelphia, Pennsylvania, U.S.A.

³Cartilage Bioengineering Laboratory, Department of Mechanical Engineering, University of Delaware, Newark, Delaware, U.S.A.

⁴Depart of Orthopaedic Surgery, University of California, San Francisco, California, U.S.A.

⁵Orthopaedic Biomechanics Laboratory, Department of Mechanical Engineering, University of California, Berkeley, California, U.S.A.

Abstract and keywords

Individual trabecula segmentation (ITS) technique can decompose the trabecular bone network into individual trabecular plates and rods and is capable of quantifying the plate/rod-related microstructural characteristics of trabecular bone. This novel technique has been shown to be able to provide in-depth insights into micromechanics and failure mechanisms of human trabecular bone, as well as to distinguish the fracture status independent of area bone mineral density in clinical applications. However, the plate/rod microstructural parameters from ITS have never been correlated to experimentally determined mechanical properties of human trabecular bone. In this study, on-axis cylindrical trabecular bone samples from human proximal tibia (n=30), vertebral body (n=10), and proximal femur (n=30) were harvested, prepared, scanned using micro computed-tomography (μCT), analyzed with ITS and mechanically tested. Regression analyses showed that the plate bone volume fraction (pBV/TV) and axial bone volume fraction (aBV/TV) calculated by ITS analysis correlated the best with elastic modulus (R²=0.96-0.97) and yield strength (R²=0.95-0.96). Trabecular plate-related microstructural parameters correlated highly with elastic modulus and yield strength, while most rod-related parameters were found inversely and only moderately correlated with the mechanical properties. In addition, ITS analysis also identified that trabecular bone at human femoral neck has the highest trabecular plate-related parameters while the other sites are similar with each other in terms of plate-rod microstructure.

Keywords: Individual trabecula segmentation; Trabecular bone; Mechancial properties; Microstructure

Links

DOI: 10.1016/j.jbiomech.2013.11.039.

PubMed: 24360196

PubMedCentral: PMC3925244

History

Accepted: 2013-11-24

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Cited By (21)

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2021	Feng C, Wang L, Xu P, Chu Z, Yao J, Sun W, Gong H, Zhang X, Li Z, Fan Y. Microstructural and mechanical evaluations of region segmentation methods in classifications of osteonecrosis. J Biomech. April 15, 2021;119:110208.
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2018	Hunt HB. Characterization of Material Properties, Microarchitecture, and Mechanics of Bone from Subjects With Type 2 Diabetes Mellitus [PhD thesis]. Ithaca, NY: Cornell University; December 2018.
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