Changes in tensile strength characteristics and histology of rabbit ligaments induced by different modes of postmortal storage

Viidik, A.; Lewin, T.

Affiliations

¹Department of Human Anatomy, University of Gothenburg, Sweden

Abstract (English)

The influence of the following modes of postmortal storage on tensile strength characteristics and histology of rabbit ligaments were studied: (A) in saline for 5 hours +20°C; (B) in saline for 24 hours +4°C; (C) deep-freezing; (D) in 10 per cent formaldehyde. In each group 8 knees were included. The fresh control material consisted of 14 knees.

The following parameters were employed: (i) the gross shape of the load-elongation curve, (ii) its slope (tan α), (iii) its failure energy, (iv) failure load, (v) elongation at failure, (vi) dips in the recorder's load curve, and (vii) failure site on the bone-ligament-bone preparation.

Standard histological methods were used.

None of the modes of storage proved satisfactory for the preservation of the tensile strength characteristics of the fresh soft connective tissue.

Only storing in formaldehyde (in fact fixing in it) proved satisfactory for histological studies.

These findings are discussed and it is concluded that biomechanical tests on collagenous tissue removed at surgery must be performed with no time lapse.

Abstract (French)

L'influence des modes suivants de stockage post-mortel sur les caractCristiques de la force de tension et I'histologie des ligaments de lapins a été étudiés: (A) dans l'eau salée pendant 5 heurcs +20°C (B) dans l'eau sake pendant 24 heures +4°C; (C) à l'état surgelé; (D) dans 10 pour cent formaldéhyde. Chaque groupe comprenait huit genoux. Le matériel frais de contrôle consistait en 14 genoux.

Les paramètres suivants ont été utilisés : (i) forme approximative de la courbe d’élongation en charge, (ii) son inclinaison (tang. α), (iii) son énergie de rupture, (iv) sa charge de rupture, (v) élongation à la rupture, (vi) chute de la courbc de charge de l’enregistreur et (vii) emplacement de la rupture sur la préparation os-ligament-os.

Des méthodes histologiques ont été utilisées.

Aucunc des méthodes de stockage ne s’est montrée satisfaisante pour la conservation des caractéristiqucs de la force de tension des tissus conjonctifs mous frais.

Seule la conservation dans la formaldéhyde (en fait la fixation dans celle-ci) s’est montrée satisfaisante pour les études histologiques.

Il est discuté de ces trouvailles et il est conch que le test biomécanique du tissu collagbne extirpé chirurgicalement doit être pratiqué sans délai.

Abstract (German)

Der Einfluss folgender Arten postmortaler Aufbewahrung auf Dehnberkeitsstärke und Histologic von Kaninchenligamenten wurde untersucht: (A) In Salzauflösung für 5 Stundcn +20°C, (H) in Salzauflösung für 24 Stunden +4°C, (C) Tieffrierung, (D) in 10 Pro zent Formaldehyd. Jede Gruppe umfasste 8 Knicee. Des frische Kontrollmaterial bestand aus 14 Knieen.

Die folgende Parameter wurden angewendet : (i) Die grobe Form der Relastungs-Verlangerungskurvc, (ii) ihr Ahfall ( Tang. o), (iii) ihre Versagungsenergie, (iv) Versagungsbelastung, (v) Verlängerung bei der Versagung, (vi) Einkerbungen in der Bclastungskurve des Rekorders, und (vii) Sitz des Nachgebens am Knochen-Ligament-Knochen Präparat.

Normierte histologische Methoden wurden verwendet.

Diese Befunde werden besprochen und man schliesst, dass biochernische Untersuchungen an kollagenem Gewebe, das anlässlich chirurgischer Eingriffe entfernt wird, ohne Zeitverlust ausgcführt werden müssen

Links

DOI: 10.3109/17453676608993274

PubMed: 5911489

WoS: A19668079700004

History

Received: 1965-08-20

Cited By (41)

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