A second rank symmetric tensor which describes the degree of orientation in orthotropic materials is presented and shown to reflect accurately patterns of experimental data. The use of this tensor to describe microstructural anisotropy is compared to currently accepted methods and is found to be more useful and accurate in experimental studies. A method for determining the anisotropy tensor in a material is given, based on measurements on any three mutually perpendicular planes, and the fundamental restriction of this method to orthotropic materials is discussed. Experimentally determined anisotropy tensors in five specimens of cancellous bone from five different human bones are given.