Three‐dimensional nuclear magnetic resonance microimaging of trabecular bone

Chung, Hsiao‐Wen<sup>1,2</sup>; Wehrli, Felix W.<sup>1</sup>; Williams, John L.<sup>3</sup>; Wehrli, Suzanne L.<sup>4</sup>

Affiliations

¹Department of Radiology, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania

²Present address: 448 Department of Electrical Engineering, National Taiwan University, Taipei, Taiwan, R.O.C.

³Department of Orthopedic Surgery, University of Pennsylvania Medical Center, Philadelphia, Pennsylvania

⁴Joseph Stokes Jr. Research Center, Children's Hospital of Philadelphia, Philadelphia, Pennsylvania

Abstract

The conventional approach to measuring structural parameters in trabecular bone rests on stereology from optical images, derived from sections of embedded bone. In order to provide data that are statistically representative of a sufficiently large volume, multiple sections need to be analyzed in each of the three orthogonal planes. In this work, an alternative technique is presented which is based on three‐dimensional (3D) volumetric proton nuclear magnetic resonance (NMR) microimaging. The method presented provides images from 9 × 9 × 4 mm³ volumes of defatted bone specimens in 15–20 minutes scan time at isotropic resolution corresponding to (78 μm)³ voxel size. Surface‐rendered images of bovine and human trabecular bone are shown and an algorithm was developed and implemented for determining the orientation and magnitude of the principal axes of the mean intercept length tensor.

Links

DOI: 10.1002/jbmr.5650101005

PubMed: 8686500

WoS: A1995RX78000004

History

Received: 1994-08-22

Revised: 1995-05-15

Accepted: 1995-06-15

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Cited By (14)

Year	Entry
2002	Wehrli F, Saha P, Gomberg B, Song H, Snyder P, Benito M, Wright A, Weening R. Role of magnetic resonance for assessing structure and function of trabecular bone. Top Magn Reson Imaging. October 2002;13(5):335-355.
1997	Beck JD, Canfield BL, Haddock SM, Chen TJH, Kothari M, Keaveny TM. Three-dimensional imaging of trabecular bone using the computer numerically controlled milling technique. Bone. 1997;21(3):281-287.
1998	Majumdar S, Kothari M, Augat P, Newitt DC, Link TM, Lin JC, Lang T, Lu Y, Genant HK. High-resolution magnetic resonance imaging: three-dimensional trabecular bone architecture and biomechanical properties. Bone. May 1998;22(5):445-454.
1999	Genant HK, Gordon C, Jiang Y, Lang TF, Link TM, Majumdar S. Advanced imaging of bone macro and micro structure. Bone. July 1999;25(1):149-152.
1997	Uchiyama T, Tanizawa T, Muramatsu H, Endo N, Takahashi HE, Hara T. A morphometric comparison of trabecular structure of human ilium between microcomputed tomography and conventional histomorphometry. Calcif Tiss Int. December 1997;61(6):493-498.
1997	Majumdar S, Genant HK. High resolution magnetic resonance imaging of trabecular structure. Eur Radiol. February 1997;7(suppl 2):S51-S55.
1998	Link TM, Majumdar S, Lin JC, Newitt D, Augat P, Ouyang X, Mathur A, Genant HK. A comparative study of trabecular bone properties in the spine and femur using high resolution mri and ct. J Bone Miner Res. January 1998;13(1):122-132.
1997	Hwang SN, Wehrli FW, Williams JL. Probability‐based structural parameters from three‐dimensional nuclear magnetic resonance images as predictors of trabecular bone strength. Med Phys. August 1997;24(8):1255-1261.
1999	Majumdar S, Link TM, Augat P, Lin JC, Newitt D, Lan NE, Genant HK. Trabecular bone architecture in the distal radius using magnetic resonance imaging in subjects with fractures of the proximal femur. Osteoporos Int. 1999;10(3):231-239.
2002	Newitt DC, van Rietbergen B, Majumdar S. Processing and analysis of in vivo high-resolution MR images of trabecular bone for longitudinal studies: reproducibility of structural measures and micro-finite element analysis derived mechanical properties. Osteoporos Int. 2002;13(4):278-287.
1998	Wehrli FW, Hwang SN, Ma J, Song HK, Ford JC, Haddad JG. Cancellous bone volume and structure in the forearm: noninvasive assessment with MR microimaging and image processing. Radiology. February 1998;206(2):347-357.
1998	Majumdar S. A review of magnetic resonance (MR) imaging of trabecular bone micro-architecture: contribution to the prediction of biomechanical properties and fracture prevalence. Technol Health Care. 1998;6(5-6):321-327.
2007	Schneider P. Ultrastructural Phenotyping of Murine Bone Using Synchrotron Micro- and Nano-Computed Tomography [PhD thesis]. Swiss Federal Institute of Technology Zürich; 2007.
2003	Fox JC. Biomechanics of the Proximal Femur: Role of Bone Distribution and Architecture [PhD thesis]. Berkeley, CA: Berkeley, University of California; 2003.