Applications of fourier transform infrared (FT-IR) microscopy to the study of mineralization in bone and cartilage

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Boskey, Adele L.¹; Pleshko, Nancy²; Doty, Stephen B.¹; Mendelsohn, Richard¹

Applications of fourier transform infrared (FT-IR) microscopy to the study of mineralization in bone and cartilage

Cell Mater. 1992;2(3):209-220

Affiliations

¹The Laboratory for Ultrastructural Biochemistry, The Hospital for Special Surgery, New York, NY

²The Department of Chemistry, Rutgers University, Newark NJ
Abstract and keywords

Knowledge of the phase, composition, and crystallite size and perfection of the mineral in normal and abnormally calcified tissues provides insight into the mechanism by which this mineral was deposited. These data also can be used to develop rational therapies for pathological conditions characterized by abnormal mineral deposition. As illustrated in this review, coupling of an optical microscope with a Fourier transform infrared (FT-IR) spectrophotometer permits the mapping at 20 μm spatial resolution of changes in mineral characteristics (content, particle size, composition) in the growth plate, in bone biopsies, in mineralizing cell culture systems, and in soft tissue calcifications. Based on the infrared properties of apatitic compounds, and comparisons with x-ray diffraction data, correlations have been established from which mineral parameters can be determined. The validity of these spectral correlations has been demonstrated by independent measurements of mineral content (ash weight), and crystal particle size (dark field electron microscopy).

Keywords: Fourier transform infrared (FT-IR); infrared spectroscopy; hydroxyapatite; calcified cartilage; bone mineral; FT-IR microscopy; cell culture; osteoporosis
Links

WoS: A1992KC00200004
History

Received: 1992-05-09

Revised: 1992-10-30

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1999	Camacho NP, Rinnerthaler S, Paschalis EP, Mendelsohn R, Boskey AL, Fratzl P. Complementary information on bone ultrastructure from scanning small angle X-ray scattering and Fourier-transform infrared microspectroscopy. Bone. September 1999;25(3):287-293.
2011	Paschalis EP, Tatakis DN, Robins S, Fratzl P, Manjubala I, Zoehrer R, Gamsjaeger S, Buchinger B, Roschger A, Phipps R, Boskey AL, Dall'Ara E, Varga P, Zysset P, Klaushofer K, Roschger P. Lathyrism-induced alterations in collagen cross-links influence the mechanical properties of bone material without affecting the mineral. Bone. December 2011;49(6):1232-1241.
2020	Taylor EA, Donnelly E. Raman and Fourier transform infrared imaging for characterization of bone material properties. Bone. October 2020;139:115490.
2020	Rokidi S, Andrade VF, Borba V, Shane E, Cohen A, Zwerina J, Paschalis EP, Moreira CA. Bone tissue material composition is compromised in premenopausal women with type 2 diabetes. Bone. December 2020;141:115634.
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2021	Hunt HB, Miller NA, Hemmerling KJ, Koga M, Lopez KA, Taylor EA, Sellmeyer DE, Moseley KF, Donnelly E. Bone tissue composition in postmenopausal women varies with glycemic control from normal glucose tolerance to type 2 diabetes mellitus. J Bone Miner Res. February 2021;36(2):334-346.
2022	Fratzl-Zelman N, Hartmann MA, Gamsjaeger S, Rokidi S, Paschalis EP, Blouin S, Zwerina J. Bone matrix mineralization and response to burosumab in adult patients with X-linked hypophosphatemia: results from the phase 3, single-arm international trial. J Bone Miner Res. September 2022;37(9):1665-1678.
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