FTIR Spectroscopy was used to study the effect of collagen and microgravity on the mineralization of osteoblast like cells in culture. Exogenously supplied collagen is necessary for cells to attach to the tissue culture substrates but did not appear to increase the rate at which the cells were able to mineralize. Absorbance bands at 1024 cm^-1 and 1038 cm^-1 wavenumbers in second derivative enhanced spectra were used to detect the presence of hydroxyapatite in the osteoblast like cells in both tissue culture polystyrene system and in rotary cell culture system. These studies confirmed the previous observations that the simulated microgravity in the rotary cell culture system accelerated the formation of hydroxyapatite, and ascorbic acid and p-glycerol phosphate are both necessary for the process of mineralization. Fourier transform infrared spectroscopy with spectral enhancement methods like second derivatives, fourier self-deconvolution and curve fitting can be used to follow the mineralization of cells in culture.