Bone quality in an ovariectomized monkey animal model treated with two doses of teriparatide for either 18 months, or 12 months followed by withdrawal for 6 months

Paschalis, E.P.; Gamsjaeger, S.; Burr, D.B.

Affiliations

¹Ludwig Boltzmann Institute for Osteology, at the Hanusch Hospital of OEGK and AUVA Trauma Centre Meidling, 1st Medical Department, Hanusch Hospital, Vienna, Austria

²Department of Anatomy and Cell Biology, Indiana University School of Medicine, Indianapolis, IN 46202, USA

³Department of Biomedical Engineering, Indiana University-Purdue University, Indianapolis (IUPUI), Indianapolis, IN 46202, USA

Abstract and keywords

Previous studies of ovariectomized (OVX) monkeys, treated with recombinant human parathyroid hormone (PTH) (1–34) at 1 or 5 μg/kg/day for 18 months or for 12 months followed by 6 months withdrawal from treatment, displayed significant changes in geometry, histomorphometry, and bone quality, but without strict tissue age criteria, of the midshaft humerus. Since bone quality significantly depends on tissue age among other factors, the aim of the present study was to establish the bone-turnover independent effects of two doses of PTH, as well as the effects of treatment withdrawal on bone quality by measuring bone material composition at precisely known tissue ages ranging from osteoid, to mineralized tissue older than 373 days. Raman microspectroscopic analysis of bone tissue from the mid-shaft humerus of OVX monkeys demonstrated that the clinically relevant dose of PTH administered for 18 months reverses the effects of ovariectomy on bone quality when compared against SHAM. Both doses investigated in this study restore the mineralization regulation mechanisms to SHAM levels. The study also showed that the beneficial effects induced by 12 months of clinically relevant PTH therapy were sustained after six months of therapy withdrawal.

Keywords: Raman microspectroscopic analysis; Bone quality; Recombinant human parathyroid hormone (1–34); Osteoporosis; Ovariectomized monkey

Links

DOI: 10.1016/j.bone.2022.116366

PubMed: 35167989

WoS: 000773553300009

History

Received: 2021-12-14

Revised: 2022-01-27

Accepted: 2022-02-8

Online: 2022-02-12

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Cited By (1)

Year	Entry
2023	Monzem S, Valkani D, Evans LAE, Chang Y-M, Pitsillides AA. Regional modular responses in different bone compartments to the anabolic effect of PTH (1-34) and axial loading in mice. Bone. May 2023;170:116720.