Crystal-collagen relationships in calcified turkey leg tendons visualized by selected-area dark field electron microscopy

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Arsenault, A. Larry

Crystal-collagen relationships in calcified turkey leg tendons visualized by selected-area dark field electron microscopy

Calcif Tiss Int. October 1988;43(4):202-212

©1988 Springer-Verlag New York Inc.

Affiliations

¹Department of Anatomy, University of British Columbia, Vancouver, Canada
Abstract and keywords

The distribution and orientation of biological apatite crystals in calcified turkey leg tendons were studied by selected-area dark field electron microscopy. This imaging technique enables the direct visualization of apatite and the specific determination of the crystallographic axes (a, b-axes or c-axis) within calcified collagen fibrils. This study shows that at early stages of mineralization, rod-shaped apatite crystals (5–20 nm in length) were localized and dispersed within gap zones bordering both the collagen molecule C- and N-terminal regions. At later stages of mineral deposition the crystals were more extensive, occupying greater areas of the gap zone and, in addition, apatite crystals were found to occur in the overlap zones. The orientation of apatite crystals was observed to be an alternating and interlocking distribution of a, b-axes and c-axis along the axial period of collagen fibrils. This distribution is interpreted as representing a continuous rotation of apatite axial orientation along the collagen period.

Keywords: Biological apatite; Crystallographic orientations; Calcified turkey leg tendon; Crystal-collagen relationships; Selected-area dark field imaging
Links

DOI: 10.1007/BF02555136

PubMed: 3145125

WoS: A1988Q167100003
History

Received: 1987-11-25

Revised: 1988-01-29

Cited Works (2)

Year	Entry
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Cited By (25)

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1992	Zylberberg L, Géraudie J, Meunier F, Sire J-Y. Biomineralization in the integumental skeleton of the living lower vertebrates. In: Hall BK, ed. Bone. Volume 3: Bone Metabolism and Mineralization. Boca Raton, FL: Inc., CRC Press; 1992:171-.
2002	Benezra Rosen V, Hobbs LW, Spector M. The ultrastructure of anorganic bovine bone and selected synthetic hyroxyapatites used as bone graft substitute materials. Biomaterials. February 2002;23(3):921-928.
1999	Wenk H-R, Heidelbach F. Crystal alignment of carbonated apatite in bone and calcified tendon: results from quantitative texture analysis. Bone. April 1999;24(4):361-369.
2003	Su X, Sun K, Cui FZ, Landis WJ. Organization of apatite crystals in human woven bone. Bone. February 2003;32(2):150-162.
2003	Rubin MA, Jasiuk I, Taylor J, Rubin J, Ganey T, Apkarian RP. TEM analysis of the nanostructure of normal and osteoporotic human trabecular bone. Bone. March 2003;32(3):270-282.
2020	Schwarcz HP, Binkley DM, Luo L, Grandfield K. A search for apatite crystals in the gap zone of collagen fibrils in bone using dark-field illumination. Bone. June 2020;135:115304.
1991	Arsenault AL. Image analysis of collagen-associated mineral distribution in cryogenically prepared turkey leg tendons. Calcif Tiss Int. January 1991;48(1):56-62.
1994	Hasegawa K, Turner CH, Burr DB. Contribution of collagen and mineral to the elastic anisotropy of bone. Calcif Tiss Int. November 1994;55(5):381-386.
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2003	Tong W, Glimcher MJ, Katz JL, Kuhn L, Eppell SJ. Size and shape of mineralites in young bovine bone measured by atomic force microscopy. Calcif Tiss Int. May 2003;72(5):592-598.
2012	Spiesz EM, Roschger P, Zysset PK. Influence of mineralization and microporosity on tissue elasticity: experimental and numerical investigation on mineralized turkey leg tendons. Calcif Tiss Int. 2012;90(4):319-329.
1992	Boskey AL, Pleshko N, Doty SB, Mendelsohn R. Applications of fourier transform infrared (FT-IR) microscopy to the study of mineralization in bone and cartilage. Cell Mater. 1992;2(3):209-220.
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2008	Ruppel ME, Miller LM, Burr DB. The effect of the microscopic and nanoscale structure on bone fragility. Osteoporos Int. September 2008;19(9):1251-1265.
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2014	Klosowski MM. Electron Microscopy Characterisation of in Vivo Collagen and Mineral Ultra-Structures, Their Development and Pathologies [PhD thesis]. Imperial College London; August 2014.
2009	Ruppel ME. Alterations in the Mineral and Collagen Matrix of Bisphosphonate-Treated Bone and Osteoblasts [PhD thesis]. Stony Brook, NY: Stony Brook University; May 2009.
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2012	Barkaoui A. Modélisation multiéchelle du comportement mécano-biologique de l’os humain: De l’ultrastructure au remodelage osseux [PhD thesis]. University of Orleans; 2012.
2010	Wynnyckyj C. The Consequences of Collagen Degradation on Bone Mechanical Properties [PhD thesis]. University of Toronto; 2010.