A comparative analysis of streaming potentials in vivo and in vitro

Otter, Mark W.<sup>1</sup>; Palmieri, Vincent R.<sup>1</sup>; Wu, Dadong D.<sup>1</sup>; Seiz, Kristin G.<sup>1</sup>; MacGinitie, Laura A.<sup>1</sup>; Cochran, George Van B.<sup>1,2</sup>

Affiliations

¹Orthopaedic Engineering and Research Center, Helen Hayes Hospital, West Haverstraw, New York

²Department of Orthopaedic Surgery, Columbia University, New York, New York, U.S.A.

Abstract and keywords

Streaming potentials (SPs) measured in vivo at a specific site on intact cortical bone (canine tibia) have been compared with measurements from the same site in vitro, tested as an excised bone strip soaked in Hank's balanced salt solution. The amplitude of SPs per periosteal strain in vitro was larger in 13 tibias than in vivo (by an average ×6.5 at 1 Hz), but values per transcortical strain difference were similar. In vitro, SP magnitudes rose more sharply to an asymptotic value as a function of bending frequency than did in vivo signals, possibly because of a difference in the internal state of canaliculi and/or Haversian systems. Similarly, SP response to step-loading decreased to zero more slowly with time in vitro than in vivo. Difficulties encountered in preliminary measurements due to electrical shunting through electrolyte and soft tissues suggest the need for caution in using both in vivo and in vitro SP measurements to extrapolate to electric field strengths on the cellular level.

Keywords: Bioelectricity; Bone; Streaming potential; Strain-related potentials

Links

DOI: 10.1002/jor.1100100513

PubMed: 1500983

WoS: A1992JJ89600012

History

Received: 1991-05-24

Accepted: 1992-04-17

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