Elevated microdamage spatially correlates with stress in metastatic vertebrae

Atkins, Ayelet; Burke, Mikhail; Samiezadeh, Saeid; Akens, Margarete K.; Hardisty, Michael; Whyne, Cari M.

Affiliations

¹Orthopaedics Biomechanics Laboratory, Sunnybrook Research Institute, Toronto, Canada

²Division of Orthopaedics, Department of Surgery, University of Toronto, Toronto, Canada

³Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, Canada

⁴Techna Institute, University Health Network, Toronto, Canada

⁵Department of Medical Biophysics, University of Toronto, Toronto, Canada

Abstract and keywords

Metastasis of cancer to the spine impacts bone quality. This study aims to characterize vertebral microdamage secondary to metastatic disease considering the pattern of damage and its relationship to stress and strain under load. Osteolytic and mixed osteolytic/osteoblastic vertebral metastases were produced in athymic rats via HeLa cervical or canine Ace-1 prostate cancer cell inoculation, respectively. After 21 days, excised motion segments (T12–L2) were µCT scanned, stained with BaSO4 and re-imaged. T13–L2 motion segments were loaded in axial compression to induce microdamage, re-stained and re-imaged. L1 (loaded) and T12 (unloaded) vertebrae were fixed, sample blocks cut, polished and BSE imaged. µFE models were generated of all L1 vertebrae with displacement boundary conditions applied based on the loaded µCT images. µCT stereological analysis, BSE analysis and µFE derived von Mises stress and principal strains were quantitatively compared (ANOVA), spatial correlations determined and patterns of microdamage assessed qualitatively. BaSO4 identified microdamage was found to be spatially correlated with regions of high stress in µFEA. Load-induced microdamage was shown to be elevated in the presence of osteolytic and mixed metastatic disease, with diffuse, crossed hatched areas of microdamage present in addition to linear microdamage and microfractures in metastatic tissue, suggesting diminished bone quality.

Keywords: Microdamage; Vertebral metastasis; Bone quality; Barium sulfate staining; Micro computed tomography; Backscatter electron imaging; Finite element analysis

Links

DOI: 10.1007/s10439-018-02188-8

PubMed: 30673956

WoS: 000461026400006

History

Received: 2018-08-03

Accepted: 2018-12-11

Online: 2019-01-23

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Cited By (1)

Year	Entry
2020	Whyne CM, Ferguson D, Clement A, Rangrez M, Hardisty M. Biomechanical properties of metastatically involved osteolytic bone. Curr Osteoporos Rep. December 2020;18(6):705-715.