Three‐dimensional morphometry of the L6 vertebra in the ovariectomized rat model of osteoporosis:​ biomechanical implications

Kinney, J. H.; Haupt, D. L.; Balooch, M.; Ladd, A. J. C.; Ryaby, J. T.; Lane, N. E.

Affiliations

¹Department of Preventive and Restorative Dental Sciences, Division of Bioengineering and Biomaterials, University of California, San Francisco, San Francisco, California, U.S.A.

²Department of Chemical Engineering, University of Florida, Gainseville, Florida, U.S.A.

³OrthoLogic Corporation, Tempe, Arizona, U.S.A.

⁴Department of Medicine, University of California, San Francisco, San Francisco, California, U.S.A.

Abstract and keywords

This article summarizes the results of a three‐dimensional study of changes in the morphology of the L6 rat vertebra at 120 days after ovariectomy (OVX), with estrogen replacement therapy used as a positive control. Synchrotron radiation microtomography was used to quantify the structural parameters defining trabecular bone architecture, while finite‐element methods were used to explore the relationships between these parameters and the compressive elastic behavior of the vertebrae. There was a 22% decrease in trabecular bone volume (TBV) and a 19% decline in mean trabecular thickness (Tb.Th) with OVX. This was accompanied by a 150% increase in trabecular connectivity, a result of the perforation of trabecular plates. Finite‐element analysis of the trabecular bone removed from the cortical shell showed a 37% decline in the Young's modulus in compression after OVX with no appreciable change in the estrogen‐treated group. The intact vertebrae (containing its trabecular bone) exhibited a 15% decrease in modulus with OVX, but this decline lacked statistical significance. OVX‐induced changes in the trabecular architecture were different from those that have been observed in the proximal tibia. This difference was a consequence of the much more platelike structure of the trabecular bone in the vertebra.

Keywords: trabecular bone; cortical bone; vertebra; rat model; osteoporosis; microtomography; atomic force microscopy; synchrotron radiation

Links

DOI: 10.1359/jbmr.2000.15.10.1981

PubMed: 11028451

WoS: 000089558700015

History

Received: 1999-09-17

Revised: 2000-05-10

Accepted: 2000-06-16

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