The generation of enlarged eroded pores upon existing intracortical canals is a major contributor to endocortical trabecularization

Andreasen, Christina Møller<sup>1,2,3</sup>; Bakalova, Lydia Peteva<sup>4</sup>; Brüel, Annemarie<sup>5</sup>; Hauge, Ellen Margrethe<sup>6</sup>; Kiil, Birgitte Jul<sup>7</sup>; Delaisse, Jean-Marie<sup>2,3,8</sup>; Kersh, Mariana Elizabeth<sup>4</sup>; Thomsen, Jesper Skovhus<sup>5</sup>; Andersen, Thomas Levin<sup>2,3,8,9</sup>

Affiliations

¹Department of Orthopedic Surgery & Traumatology, Odense University Hospital, Odense, Denmark

²Clinical Cell Biology, Research Unit of Pathology, Department of Clinical Research, University of Southern Denmark and Department of Pathology, Odense University Hospital, Odense, Denmark

³Department of Molecular Medicine, University of Southern Denmark, Odense, Denmark

⁴Department of Mechanical Science and Engineering, University of Illinois at Urbana-Champaign, IL, USA

⁵Department of Biomedicine, Aarhus University, Aarhus, Denmark

⁶Department of Rheumatology, Aarhus University Hospital and Department of Clinical Medicine, Aarhus University, Aarhus, Denmark

⁷Department of Plastic Surgery, Aarhus University Hospital, Aarhus, Denmark

⁸Clinical Cell Biology, Vejle Hospital – Lillebælt Hospital, University of Southern Denmark, Vejle, Denmark

⁹Department of Forensic Medicine, Aarhus University, Aarhus, Denmark

Abstract and keywords

The gradual conversion of cortical bone into trabecular bone on the endocortical surface contributes substantially to thinning of the cortical bone. The purpose of the present study was to characterize the intracortical canals (3D) and pores (2D) in human fibular bone, to identify the intracortical remodeling events leading to this endocortical trabecularization. The analysis was conducted in fibular diaphyseal bone specimens obtained from 20 patients (6 women and 14 men, age range 41–75 years). μCT revealed that endosteal bone had a higher cortical porosity (p < 0.05) and canals with a larger diameter (p < 0.05) than periosteal bone, while the canal spacing and number were similar in the endosteal and periosteal half. Histological analysis showed that the endosteal half versus the periosteal half: (i) had a higher likelihood of being non-quiescent type 2 pores (i.e. remodeling of existing pores) than other pore types (OR = 1.6, p < 0.01); (ii) that the non-quiescent type 2 pores contributed to a higher porosity (p < 0.001); and that (iii) amongst these pores especially eroded type 2 pores contributed to the elevated cortical porosity (p < 0.001).

In conclusion, we propose that endocortical trabecularization results from the accumulation of eroded cavities upon existing intracortical canals, favored by delayed initiation of bone formation.

Keywords: Cortical porosity; Bone remodeling; Bone resorption; Bone formation; Trabecularization

Links

DOI: 10.1016/j.bone.2019.115127

PubMed: 31689525

WoS: 000503321100028

History

Received: 2019-06-19

Revised: 2019-10-28

Accepted: 2019-10-29

Online: 2019-11-02

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Cited By (3)

Year	Entry
2022	Lamarche BA, Thomsen JS, Andreasen CM, Lievers WB, Andersen TL. 2D size of trabecular bone structure units (BSU) correlate more strongly with 3D architectural parameters than age in human vertebrae. Bone. July 2022;160:116399.
2022	Borggaard XG, Roux J-P, Delaisse J-M, Chavassieux P, Andreasen CM, Andersen TL. Alendronate prolongs the reversal-resorption phase in human cortical bone remodeling. Bone. July 2022;160:116419.
2022	van Dijk Christiansen P. The Relevance of Reversal Cells and Intermittent Parathyroid Hormone on the Duration of the Reversal-Resorption Phase in Intracortical Bone Remodeling Events [PhD thesis]. University of Southern Denmark (SDU); November 2022.