The Development of a Digital X-Ray Imaging System for the Characterization of Transmission of Whole-Body Vibration in Mice

Mechanical stimulus such as whole-body vibration (WBV] has shown to promote bone formation both in humans and animals. WBV has been hypothesized as a potentially useful osteoporosis intervention. While the transmission of WBV has been well characterized in humans, there is currently a lack of knowledge in the transmission of vibration in small animal models, such as mice, due to the lack of an implantable accelerometer appropriate to make such measurements. This thesis introduces an image-based method to quantify transmission of vibration in mice using x-ray imaging. Specifically, it utilizes motion blur of fiducial markers, which are implanted into the mouse tibia and femur. Vibration characteristics in vivo were characterized over the range of 15-40 Hz. Resonance was observed in the femur at 25 Hz and reduction in transmission in the tibia at 30 Hz. These findings provide an estimate of the magnitude of vibration transmitted into the animal’s limb.

Keywords: Whole-body vibration; Osteoporosis; Mice; Bone Resonance; Skeletal Loading; Tungsten Carbide Implant

Year	Entry
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Year

Entry

2004

Verschueren SMP, Roelants M, Delecluse C, Swinnen S, Vanderschueren D, Boonen S. Effect of 6‐month whole body vibration training on hip density, muscle strength, and postural control in postmenopausal women: a randomized controlled pilot study. J Bone Miner Res. March 2004;19(3):352-359.

2003

Boyle WJ, Simonet WS, Lacey DL. Osteoclast differentiation and activation. Nature. May 15, 2003;423(6937):337-342.

1994

Parfitt AM. Osteonal and hemi‐osteonal remodeling: the spatial and temporal framework for signal traffic in adult human bone. J Cell Biochem. July 1994;55(3):273-286.

1995

Riggs BL, Melton LJ III. The worldwide problem of osteoporosis: insights afforded by epidemiology. Bone. November 1995;17(5)(suppl 1):S505-S511.

1994

Kanis JA; WHO Study Group. Assessment of fracture risk and its application to screening for postmenopausal osteoporosis: synopsis of a WHO report. Osteoporos Int. November 1994;4(6):368-381.