Hydration effects on the micro-mechanical properties of bone

Bembey, A. K.; Bushby, A. J.; Boyde, A.; Ferguson, V. L.; Oyen, M. L.

Affiliations

¹Department of Materials, Queen Mary, University of London, London E1 4NS, United Kingdom

²Biophysics Section, Centre for Oral Growth and Development, Dental Institute, Queen Mary, University of London, London E1 1BB, United Kingdom

³Department of Mechanical Engineering, University of Colorado, Boulder, Colorado 80309

⁴Center for Applied Biomechanics, Department of Mechanical and Aerospace Engineering, University of Virginia, Charlottesville, Virginia 22902

Abstract

Bone is a composite material with hydroxyapatite mineral, collagen, and water as primary components. Water plays an important role in maintaining the mechanical integrity of the composite, but the manner in which water interacts within the collagen and mineral at ultrastructural length-scales is poorly understood. The current study examined changes in the mechanical properties of bone as a function of hydration state. Tissues were soaked in different solvents and solutions, with different polarities, to manipulate tissue hydration. Mineralized bone was characterized using nanoindentation creep tests for quantification of both the elastic and viscoelastic mechanical responses, which varied dramatically with tissue bathing solution. The results were considered within the context of solution physical chemistry. Selectively removing and then replacing water provided insights into the ultrastructure of the tissues via the corresponding changes in the experimentally determined mechanical responses.

Links

DOI: 10.1557/JMR.2006.0237

WoS: 000239674800011

History

Received: 2006-01-11

Accepted: 2006-02-15

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