2D and 3D mapping of microindentations in hydrated and dehydrated cortical bones using confocal laser scanning microscopy

Yin, Ling<sup>1</sup>; Venkatesan, Sudharshan<sup>2</sup>; Webb, Daryl<sup>3</sup>; Kalyanasundaram, Shankar<sup>2</sup>; Qin, Qing-Hua<sup>2</sup>

Affiliations

¹School of Engineering & Physical Sciences, James Cook University, Townsville, QLD, 4811, Australia

²School of Engineering, Australian National University, Canberra, ACT, 0200, Australia

³Electron Microscopy Unit, Australian National University, Canberra, ACT, 0200, Australia

Abstract

We report on responses of hydrated and dehydrated cortical bone tissues to mechanical loading applied by a Vickers indenter. The Vickers indentations were imaged in two- and three-dimensions (2D and 3D) using confocal laser scanning microscopy (CLSM) to understand mechanical behavior of bone tissues. Serial optical sections of indentation patterns of dry and wet bones were collected using CLSM. The indention surface structures were mapped using topographical CLSM imaging. The observation of CLSM shows the fundamental indentation responses for both the hydrated and dehydrated bone tissues were plastic deformation. No visible fracture was observed in the Vickers indentation patterns in the wet bone tissue, while non-propagating lamellar microcracks occurred in the dry bone tissue. This indicates that drying resulted in increased brittleness of the bone tissue. The Vickers hardness values of dry bone tissue were significantly higher than those of wet bone tissue at any applied loads (analysis of variation, ANOVA, p < 0.05). The resolution limits of confocal microscopy were also discussed for bone tissue scanning.

Links

DOI: 10.1007/s10853-012-6301-x

WoS: 000302242700024

History

Received: 2011-11-08

Accepted: 2012-01-25

Online: 2012-02-14

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Year	Entry
2015	Granke M, Does MD, Nyman JS. The role of water compartments in the material properties of cortical bone. Calcif Tiss Int. September 2015;97(3):292-307.
2021	Karali A, Kao AP, Zekonyte J, Blunn G, Tozzi G. Micromechanical evaluation of cortical bone using in situ XCT indentation and digital volume correlation. J Mech Behav Biomed Mater. March 2021;115:104298.
2014	Carretta R. Post-Yield Mechanics and Material Composition of Single Trabeculae: A Combined Experimental and Modelling Approach [PhD thesis]. Swiss Federal Institute of Technology Zürich; 2014.
2020	Karali A. Multi-Scale Evaluation of Bone Combining Indentation, in Situ XCT Mechanics and Digital Volume Correlation [PhD thesis]. Portsmouth, England: University of Portsmouth; 2020.