The anatomic complexity and heterogeneity of the human abdomen make it difficult to develop a biofidelic abdominal component in an anthropomorphic test device that can predict abdominal injuries in crash testing. Several previous studies have attempted to relate external physical parameters such as energy, external pressure, velocity, and compression to abdominal injury. While many of these approaches have been useful in developing response corridors for the abdomen as a whole, the interaction between different abdominal organs and the responses of individual organs are not well understood. The objective of this study is to develop and test a methodology for measuring intravascular pressure gradients within isolated, physiologically pressurized liver specimens in response to blunt impact. A long-term objective will be to investigate whether a relationship exists between measured intravascular pressure gradients and liver injury in impact tests of isolated liver specimens and post-mortem human subjects. Experimental methods include the application of fluid-filled catheters as intravascular pressure-measuring devices. The frequency response characteristics of a fluid-filled catheter/transducer system are presented, along with the development of a transfer function to correct potential distortion of pressure waveforms from fluid-filled catheter pressure-measuring systems. Current results indicate that in a test chamber model, good correlation exists between corrected catheter-based pressure measurements and actual local pressures at the internal catheter opening. The applicability of the transfer function in an impact test of an isolated liver specimen has also been verified.