Determination of the relationship between collagen cross-links and the bone–tissue stiffness in the porcine mandibular condyle

Willems, Nop M. B. K.; Mulder, Lars; Bank, Ruud A.; Grünheid, Thorsten; Toonder, Jaap M. J. den; Zentner, Andrej; Langenbach, Geerling E. J.

Affiliations

¹Department of Orthodontics, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Amsterdam, the Netherlands

²Department of Functional Anatomy, Research Institute MOVE, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Amsterdam, the Netherlands

³Department of Oral Cell Biology, Research Institute MOVE, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and VU University, Amsterdam, the Netherlands

⁴Department of Biomedical Engineering, Eindhoven University of Technology, Eindhoven, the Netherlands

⁵TNO Quality of Life, Leiden, the Netherlands

⁶Department of Mechanical Engineering, Eindhoven University of Technology, Eindhoven, the Netherlands

Abstract and keywords

Although bone–tissue stiffness is closely related to the degree to which bone has been mineralized, other determinants are yet to be identified. We, therefore, examined the extent to which the mineralization degree, collagen, and its cross-links are related to bone–tissue stiffness.

A total of 50 cancellous and cortical bone samples were derived from the right mandibular condyles of five young and five adult female pigs. The degree of mineralization of bone (DMB) was assessed using micro-computed tomography. Using high-performance liquid chromatography, we quantified the collagen content and the number of cross-links per collagen molecule of two enzymatic cross-links: hydroxylysylpyridinoline (HP) and lysylpyridinoline (LP), and one non-enzymatic cross-link: pentosidine (Pen). Nanoindentation was used to assess bone–tissue stiffness in three directions, and multiple linear regressions were used to calculate the correlation between collagen properties and bone–tissue stiffness, with the DMB as first predictor.

Whereas the bone–tissue stiffness of cancellous bone did not differ between the three directions of nanoindentation, or between the two age groups, cortical bone–tissue stiffness was higher in the adult tissue. After correction for DMB, the cross-links studied did not increase the explained variance. In the young group, however, LP significantly improved the explained variance in bone–tissue stiffness. Approximately half of the variation in bone–tissue stiffness in cancellous and cortical bone was explained by the DMB and the LP cross-links and thus they cannot be considered the sole determinants of the bone–tissue stiffness.

Keywords: Mineralization; Collagen; Cross-links; Nanoindentation; Mechanical properties

Links

DOI: 10.1016/j.jbiomech.2011.01.023

PubMed: 21333996

WoS: 000290187500022

History

Accepted: 2011-01-20

Online: 2011-02-18

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2019	Semaan M, Karam E, Baron C, Pithioux M. Estimation of the elastic modulus of child cortical bone specimens via microindentation. Connect Tissue Res. July 4, 2019;60(4):399-405.
2012	Feng L, Chittenden M, Schirer J, Dickinson M, Jasiuk I. Mechanical properties of porcine femoral cortical bone measured by nanoindentation. J Biomech. June 26, 2012;45(10):1775-1782.
2011	Spiesz EM. Experimental and Computational Micromechanics of Mineralized Tendon and Bone [PhD thesis]. Vienna University of Technology; October 2011.
2014	McNerny EMB. Collagen Cross-Linking as a Determinant of Bone Quality: The Importance of Cross-Linking to Mechanical Properties as Explored by Cross-Link Inhibition and Exercise [PhD thesis]. University of Michigan; 2014.
2012	Barkaoui A. Modélisation multiéchelle du comportement mécano-biologique de l’os humain: De l’ultrastructure au remodelage osseux [PhD thesis]. University of Orleans; 2012.