Micro- and nano-CT for the study of bone ultrastructure

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Peyrin, Françoise^1,2,3,4,5,6; Dong, Pei^1,2,3,4,5,6; Pacureanu, Alexandra⁶; Langer, Max^1,2,3,4,5,6

Micro- and nano-CT for the study of bone ultrastructure

Curr Osteoporos Rep. December 2014;12(4):465-474

©2014 Springer Science+Business Media New York

Affiliations

¹INSERM U1044, CREATIS, 69621 Villeurbanne, France

²INSA-Lyon, 69621 Villeurbanne, France

³CNRS UMR 5220, 69621 Villeurbanne, France

⁴Université de Lyon, 69622 Lyon, France

⁵Université Lyon 1, 69622 Villeurbanne, France

⁶European Synchrotron Radiation Facility, BP 220, 38043 Grenoble, Cedex, France
Abstract and keywords

Micro-computed tomography (micro-CT)—a version of X-ray CT operating at high spatial resolution—has had a considerable success for the investigation of trabecular bone micro-architecture. Currently, there is a lot of interest in exploiting CT techniques at even higher spatial resolutions to assess bone tissue at the cellular scale. After recalling the basic principles of micro-CT, we review the different existing system, based on either standard X-ray tubes or synchrotron sources. Then, we present recent applications of micro- and nano-CT for the analysis of osteocyte lacunae and the lacunar-canalicular network. We also address the question of the quantification of bone ultrastructure to go beyond the sole visualization.

Keywords: Computerized tomography; Synchrotron imaging; Micro-CT; Nano-CT; X-ray phase CT; Bone ultrastructure; Osteocyte lacunae; Canaliculi; Lacunar canalicular network; Bone quality; Lacunar density; Quantification
Links

DOI: 10.1007/s11914-014-0233-0

PubMed: 25292366

WoS: 000347284400011
History

Online: 2014-10-08

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