Expression of Cre recombinase in the developing mouse limb bud driven by a <em>Prxl</em> enhancer

Logan, Malcolm; Martin, James F.; Nagy, Andras; Lobe, Corrinne; Olson, Eric N.; Tabin, Clifford J.

Affiliations

¹Department of Genetics, Harvard Medical School, Boston, Massachusetts

²Alkek Institute of Biosciences and Technology, Texas A&M System Health Science Center, Houston, Texas

³Samuel Lunenfeld Research Institute, Mount Sinai Hospital, Toronto, Canada, and Department of Molecular and Medical Genetics, University of Toronto, Toronto, Canada

⁴Sunnybrook and Women's College Health Sciences Centre and Department of Medical Biophysics, University of Toronto, Toronto, Canada

⁵Department of Molecular Biology, University of Texas Southwestern, Dallas, Texas

⁶Current affiliation: National Institute for Medical Research, Mill Hill, London, NW7 1AA, UK

Abstract

We have used a Prx1 limb enhancer to drive expression of Cre Recombinase in transgenic mice. This regulatory element leads to Cre expression throughout the early limb bud mesenchyme and in a subset of craniofacial mesenchyme. Crossing a murine line carrying this transgene to a reporter mouse harboring a floxed Cre-reporter cassette revealed that recombinase activity is first observed in the earliest limb bud at 9.5 dpc. By early to mid bud stages at 10.5 dpc recombination is essentially complete in all mesenchymal cells in the limb. Expression of the Cre recombinase was never detected in the limb bud ectoderm. The use of Prx1–Cre mice should facilitate analysis of gene function in the developing limb.

Links

DOI: 10.1002/gene.10092

PubMed: 12112875

WoS: 000176280100004

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