The repair of experimentally produced defects in rabbit articular cartilage by autologous chondrocyte transplantation

Grande, Daniel A.; Pitman, Mark I.; Peterson, Lars; Menche, David; Klein, Michael

Affiliations

¹Department of Bioengineering, Hospital for Joint Diseases Orthopaedic Institute, New York, New York, U.S.A.

²Department of Sports Medicine, Hospital for Joint Diseases Orthopaedic Institute, New York, New York, U.S.A.

³Department of Orthopaedic Surgery, Eastern Hospital, Goteburg, Sweden

⁴Department of Orthopaedic Surgery, Hospital for Joint Diseases Orthopaedic Institute, New York, New York, U.S.A.

⁵Department of Pathology, Hospital for Joint Diseases Orthopaedic Institute, New York, New York, U.S.A.

Abstract and keywords

Using the knee joints of New Zealand White rabbits, a baseline study was made to determine the intrinsic capability of cartilage for healing defects that do not fracture the subchondral plate. A second experiment examined the effect of autologous chondrocytes grown in vitro on the healing rate of these defects. To determine whether any of the reconstituted cartilage resulted from the chondrocyte graft, a third experiment was conducted involving grafts with chondrocytes that had been labeled prior to grafting with a nuclear tracer. Results were evaluated using both qualitative and quantitative light microscopy. Macroscopic results from grafted specimens displayed a marked decrease in synovitis and other degenerative changes. In defects that had received transplants, a significant amount of cartilage was reconstituted (82%) compared to ungrafted controls (18%). Autoradiography on reconstituted cartilage showed that there were labeled cells incorporated into the repair matrix.

Keywords: Articular cartilage; Cartilage grafts; Wound healing

Links

DOI: 10.1002/jor.1100070208

PubMed: 2918421

WoS: A1989T300200007

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