Enhanced knowledge of the acetabulum is of paramount importance in the diagnostic, planning, and execution stages of procedures and treatments targeting the hip joint. The convoluted and highly variable morphology of the structures comprising the bony pelvis make ascertaining measures of the acetabulum challenging. Furthermore, current methods for determining acetabular orientation assume symmetry between the separate halves of the pelvis by utilizing a coordinate system based on bilateral landmarks. The purpose of this study was to determine the three-dimensional orientation of the entire acetabulum. For this research, an improved programmatic method was developed for determining acetabular orientation using three-dimensional data. Accurate measures of commonly used acetabular orientation were ascertained from a large population of normal subjects. In addition, unilateral-based measures were performed using a new “hemi-pelvis coordinate system”. Significant differences between genders were observed in both the overall orientation of the acetabulum and the structural arrangement of the innominate bone.