Comparison of chondrogensis in static and perfused bioreactor culture

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Pazzano, David¹; Mercier, Kathi A.^1,2; Moran, John M.^1,3; Fong, Stephen S.^1,3; DiBiasio, David D.³; Rulfs, Jill X.⁴; Kohles, Sean S.²; Bonassar, Lawrence J.¹

Comparison of chondrogensis in static and perfused bioreactor culture

Biotechnol Prog. September–October 2000;16(5):893-896

©2000 American Chemical Society and American Institute of Chemical Engineers

Affiliations

¹University of Massachusetts Medical School

²Department of Biomedical Engineering, Worcester Polytechnic Institute.

³Department of Chemical Engineering, Worcester Polytechnic Institute

⁴Department of Biology, Worcester Polytechnic Institute
Abstract

As a result of the low yield of cartilage from primary patient harvests and a high demand for autologous cartilage for reconstructive surgery and structural repair, primary explant cartilage must be augmented by tissue engineering techniques. In this study, chondrocytes seeded on PLLA/PGA scaffolds in static culture and a direct perfusion bioreactor were biochemically and histologically analyzed to determine the effects of fluid flow and media pH on matrix assembly. A gradual media pH change was maintained in the bioreactor within 7.4−6.96 over 2 weeks compared to a more rapid decrease from 7.4 to 6.58 in static culture over 3 days. Seeded scaffolds subjected to 1 μm/s flow demonstrated a 118% increase (p < 0.05) in DNA content, a 184% increase (p < 0.05) in GAG content, and a 155% (p< 0.05) increase in hydroxyproline content compared to static culture. Distinct differences were noted in tissue morphology, including more intense staining for proteoglycans by safranin-O and alignment of cells in the direction of media flow. Culture of chondrocyte seeded matrices thus offers the possibility of rapid in vitro expansion of donor cartilage for the repair of structural defects, tracheal injury, and vascularized tissue damage.
Links

DOI: 10.1021/bp000082v

PubMed: 11027186

WoS: 000089797600032
History

Accepted: 2000-07-18

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2006	Aufderheide AC. Mechanical Stimulation Towards Tissue Engineering of the Knee Meniscus [PhD thesis]. Houston, TX: Rice University; June 2006.
2006	Leipzig ND. Growth Factor Effects on Single Chondrocyte Biomechanics and Gene Expression [PhD thesis]. Houston, TX: Rice University; April 2006.
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2007	McMahon LA. The Effect of Cyclic Tensile Loading and Growth Factors on the Chondrogenic Differentiation of Bone-Marrow Derived Mesenchymal Stem Cells in a Collagen-Glycosaminoglycan Scaffold [PhD thesis]. Trinity College Dublin; September 2007.
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