Osteocytic osteolysis observed in rats to which parathyroid hormone was continuously administered

Tazawa, Kohei; Hoshi, Kazuto; Kawamoto, Shinichiro; Tanaka, Mikako; Ejiri, Sadakazu; Ozawa, Hidehiro

Affiliations

¹Division of Anatomy and Cell Biology of the Hard Tissue, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

²Department of Menicon Cartilage and Bone Regeneration, Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan

³Kagoshima University Graduate School of Medical and Dental Science, Kagoshima, Japan

⁴Division of Removable Prosthodontics, Department of Tissue Regeneration and Reconstruction, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan

⁵Institute of Dental Science, Matsumoto Dental University, Shiojiri, Japan

Abstract and keywords

In order to prove osteocytic osteolysis in vivo, human parathyroid hormone (hPTH (1–34), 749 ng/h), or only solvent of the same volume, was continuously administered to 8-month-old rats by an infusion pump for 4 weeks, and then structural changes in osteocytes in the cortical bones of the tibiae were analyzed morphometrically, histologically, and histochemically. Based on contact microradiography (CMR) observations, the osteocyte lacunae in the PTH group tended to be enlarged, compared with those of the control, while the average lacuna area was 137.0 µm2 in the PTH group versus 93.9 µm2 in the control, suggesting evidence of osteocytic osteolysis. Acid phosphatase enzyme histochemical localization was observed in some osteocytes in the PTH group; therefore, lysosome systems may participate in the osteolytic mechanisms. On histological samples stained with hematoxylin-eosin or toluidine blue, the lacunae of the controls were surrounded by narrow areas of matrices both positive for hematoxylin and metachromatic for toluidine blue, while belt-like areas positive for hematoxylin were observed around the PTH-group lacunae. These findings suggested that, after osteocytic osteolysis, regenerated bone matrices may be added to the walls of osteocytes that possess enlarged lacunae.

Keywords: osteocytic osteolysis; parathyroid hormone; bone; osteocyte; acid phosphatase

Links

DOI: 10.1007/s00774-004-0519-x

PubMed: 15490261

WoS: 000224617000002

History

Received: 2003-12-12

Accepted: 2004-04-30

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