A versatile shear and compression apparatus for mechanical stimulation of tissue culture explants

Frank, Eliot H.; Jin, Moonsoo; Loening, Andreas M.; Levenston, Marc E.; Grodzinsky, Alan J.

Affiliations

¹Continuum Electromechanics Group, Center for Biomedical Engineering, Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Room 38}377, Cambridge, MA 02139, USA

²Department of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA USA

Abstract and keywords

We have developed an incubator housed, biaxial-tissue-loading device capable of applying axial deformations as small as 1 μm and sinusoidal rotations as small as 0.01°. Axial resolution is 50 nm for applying sinewaves as low as 10 μm (or 1% based on a 1 mm thickness) or as large as 100 μm. Rotational resolution is 0.0005°. The machine is small enough (30 cm high × 25 cm × 20 cm) to be placed in a standard incubator for long-term tissue culture loading studies. In metabolic studies described here, application of sinusoidal macroscopic shear deformation to articular cartilage explants resulted in a significant increase in the synthesis of proteoglycan and proteins (uptake of ³⁵S-sulfate and ³H-proline) over controls held at the same static offset compression.

Keywords: Mechanotransduction; Shear; Tissue loading; Chondrocyte metabolism; Cartilage biomechanics

Links

DOI: 10.1016/S0021-9290(00)00100-7

PubMed: 10940414

WoS: 000089948900023

History

Accepted: 2000-04-14

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2006	Aufderheide AC, Athanasiou KA. A direct compression stimulator for articular cartilage and meniscal explants. Ann Biomed Eng. September 2006;34(9):1463-1474.
2000	Grodzinsky AJ, Levenston ME, Jin M, Frank EH. Cartilage tissue remodeling in response to mechanical forces. Annu Rev Biomed Eng. 2000;2:691-713.
2001	Jin M, Frank EH, Quinn TM, Hunziker EB, Grodzinsky AJ. Tissue shear deformation stimulates proteoglycan and protein biosynthesis in bovine cartilage explants. Arch Biochem Biophys. November 1, 2001;395(1):41-48.
2004	Kisiday JD, Jin M, DiMicco MA, Kurz B, Grodzinsky AJ. Effects of dynamic compressive loading on chondrocyte biosynthesis in self-assembling peptide scaffolds. J Biomech. May 2004;37(5):595-604.
2023	Szapary HJ, Flaman L, Frank E, Chubinskaya S, Dwivedi G, Grodzinsky AJ. Effects of dexamethasone and dynamic loading on cartilage of human osteochondral explants challenged with inflammatory cytokines. J Biomech. March 2023;149:111480.
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