Static compression of articular cartilage can reduce solute diffusivity and partitioning: implications for the chondrocyte biological response

wbldb

Quinn, T. M.¹; Morel, V.¹; Meister, J. J.¹

Static compression of articular cartilage can reduce solute diffusivity and partitioning: implications for the chondrocyte biological response

J Biomech. November 2001;34(11):1463-1469

©2001 Elsevier Science Ltd. All rights reserved.

Affiliations

¹Biomedical Engineering Laboratory, Swiss Federal Institute of Technology, EPFL, PSE-A, 1015 Lausanne, Switzerland
Abstract and keywords

Chondrocytes depend upon solute transport within the avascular extracellular matrix of adult articular cartilage for many of their biological activities. Alterations to bioactive solute transport may, therefore, represent a mechanism by which cartilage compression is transduced into cellular metabolic responses. We investigated the effects of cartilage static compression on diffusivity and partitioning of a range of model solutes including dextrans of molecular weights 3 and 40 kDa, and tetramethylrhodamine (a 430 Da fluorophore). New fluorescence methods were developed for real-time visualization and measurement of transport within compressed cartilage explants. Experimental design allowed for multiple measurements on individual explants at different compression levels in order to minimize confounding influences of compositional variations. Results demonstrate that physiological levels of static compression may significantly decrease solute diffusivity and partitioning in cartilage. Effects of compression were most dramatic for the relatively high molecular weight solutes. For 40 kDa dextran, diffusivity decreased significantly (p<0.01) between 8% and 23% compression, while partitioning of 3 and 40 kDa dextran decreased significantly (p<0.01) between free-swelling conditions and 8% compression. Since diffusivity and partitioning can influence pericellular concentrations of bioactive solutes, these observations support a role for perturbations to solute transport in mediating the cartilage biological response to compression.

Keywords: Cartilage; Compression; Diffusion; Partition; Dextran
Links

DOI: 10.1016/S0021-9290(01)00112-9

PubMed: 11672721

WoS: 000171955400011
History

Accepted: 2001-06-18

Cited Works (9)

Year	Entry
2000	Eckstein F, Lemberger B, Stammberger T, Englmeier KH, Reiser M. Patellar cartilage deformation in vivo after static versus dynamic loading. J Biomech. July 2000;33(7):819-825.
1970	Maroudas A. Distribution and diffusion of solutes in articular cartilage. Biophys J. May 1970;10(5):365-379.
1987	Torzilli PA, Adams TC, Mis RJ. Transient solute diffusion in articular cartilage. J Biomech. 1987;20(2):203-214.
1989	Sah RL-Y, Kim Y-J, Doong J-YH, Grodzinsky AJ, Plass AHK, Sandy JD. Biosynthetic response of cartilage explants to dynamic compression. J Orthop Res. 1989;7(5):619-636.
1975	Maroudas A. Biophysical chemistry of cartilaginous tissues with special reference to solute and fluid transport. Biorheology. 1975;12(3-4):233-248.
1997	Torzilli PA, Arduino JM, Gregory JD, Bansal M. Effect of proteoglycan removal on solute mobility in articular cartilage. J Biomech. September 1997;30(9):895-902.
1998	Quinn TM, Grodzinsky AJ, Buschmann MD, Kim YJ, Hunziker EB. Mechanical compression alters proteoglycan deposition and matrix deformation around individual cells in cartilage explants. J Cell Sci. March 1998;111(5):573-583.
1993	Burstein D, Gray ML, Hartman AL, Gipe R, Foy BD. Diffusion of small solutes in cartilage as measured by nuclear magnetic resonance (nmr) spectroscopy and imaging. J Orthop Res. July 1993;11(4):465-478.
2000	Bonassar LJ, Grodzinsky AJ, Srinivasan A, Davila SG, Trippel SB. Mechanical and physicochemical regulation of the action of insulin-like growth factor-I on articular cartilage. Arch Biochem Biophys. July 1, 2000;379(1):57-63.

Cited By (25)

Year	Entry
2004	Ferguson SJ, Ito K, Nolte L-P. Fluid flow and convective transport of solutes within the intervertebral disc. J Biomech. February 2004;37(2):213-221.
2003	Mauck RL, Hung CT, Ateshian GA. Modeling of neutral solute transport in a dynamically loaded porous permeable gel: implications for articular cartilage biosynthesis and tissue engineering. J Biomech Eng. October 2003;125(5):602-614.
2014	Stewart RC. A Diagnostic Imaging Technique and Therapeutic Strategy for Early Osteoarthritis [PhD thesis]. Boston University; 2014.
2012	Kuo J. Bioengineering Approach to Understanding TMJ Pathobiology [PhD thesis]. Clemson, SC: Clemson University; May 2012.
2012	Shi C. Develop Novel Frap Techniques for Determining Anisotropic Solute Diffusion in Cartilaginous Tissues [PhD thesis]. Clemson, SC: Clemson University; December 2012.
2015	Wright GJ. Biomechanical Characterization and Modeling of the Human TMJ Disc [PhD thesis]. Clemson, SC: Clemson University; May 2015.
2016	Cisewski SE. Nutrient Related Mechanisms of Intervertebral Disc Degeneration [PhD thesis]. Clemson, SC: Clemson University; August 2016.
2003	Mauck RL. Functional Tissue Engineering of Articular Cartilage: The Effect of Physical Forces on the in Vitro Growth of Engineered Constructs [PhD thesis]. Columbia University; 2003.
2006	Chahine NO. Multi-Scale Measurements of the Mechanical and Transport Properties of Native and Engineered Articular Cartilage [PhD thesis]. Columbia University; 2006.
2008	Lima EG. The Tissue-Engineering of Functional Osteochondral Constructs for Cartilage Repair [PhD thesis]. Columbia University; 2008.
2018	DiDomenico CD. Evaluating Macromolecular Transport in Articular Cartilage to Better Understand and Predict Transport of Arthritis Therapeutics [PhD thesis]. Ithaca, NY: Cornell University; August 2018.
2007	Leddy HA. Diffusional Properties of Articular Cartilage [PhD thesis]. Duke University; 2007.
2008	Estes BT. Functional Tissue Engineering of Cartilage Using Adipose-Derived Stem Cells [PhD thesis]. Duke University; 2008.
2005	Imler SM. In Vitro Modulation of Meniscus Biosynthesis: A Basis for Understanding Cellular Response to Physiologically Relevant Stimuli [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; August 2005.
2007	Irrechukwu ON. The Role of Matrix Composition and Age in Solute Diffusion Within Articular Cartilage [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; December 2007.
2007	Palmer AW. Investigations of the Composition-Function Relationships in Normal, Degraded, and Engineered Articular Cartilage Using Epic-Microcomputed Tomography [PhD thesis]. Atlanta, GA: Georgia Institute of Technology; May 2007.
2013	Moeini M. Solute Transport in Articular Cartilage: Toward Applications of Diffusion, Partitioning and Adsorption for Tissue Functional Assessment [PhD thesis]. McGill University; August 2013.
2013	Shafieyan Y. Mechanical Stimulation of Orthopaedic Tissues: Regulation of Bone Resorption and Solute Transport Through Articular Cartilage [PhD thesis]. McGill University; August 2013.
2004	Yao H. Physical Signals and Solute Transport in Cartilaginous Tissues [PhD thesis]. University of Miami; August 2004.
2010	Jackson AR. Transport and Metabolism of Glucose in Intervertebral Disc [PhD thesis]. University of Miami; December 2010.
2011	Yuan T-Y. Innovative Methods to Determine Material Properties of Cartilaginous Tissues and Application for Tissue Engineering [PhD thesis]. University of Miami; August 2011.
2017	Kleinhans KL. Transport Properties in Porcine Meniscus Fibrocartilage [PhD thesis]. University of Miami; August 2017.
2005	Ng LJ. Probing Nanomechanics of Aggrecan and the Aggrecan-Rich Pericellular Matrix of Chondrocytes in Cartilage [PhD thesis]. Cambridge, MA: Massachusetts Institute of Technology; September 2005.
2010	Byun S. Transport of Proteins, Biopharmaceuticals and Small Pharmaceutical Compounds Into Normal and Injured Cartilage [PhD thesis]. Cambridge, MA: Massachusetts Institute of Technology; June 2010.
2016	Young WT. Cartilage Stress Relaxation Induced by Intra-Tissue Transport of Cationic Nanoparticles: Implications for Post-Traumatic Osteoarthritis Drug Delivery [Master's thesis]. Cambridge, MA: Massachusetts Institute of Technology; June 2016.