Anterior longitudinal ligament (ALL) injuries following whiplash have been documented both in vivo and in vitro;​ however, ALL strains during the whiplash trauma remain unknown. A new in vitro whiplash model and a bench-top trauma sled were used in an incremental trauma protocol to simulate whiplash at 3.5, 5, 6.5 and 8 g accelerations, and peak ALL strains were determined for each trauma. Following the final trauma, the ALLs were inspected and classified as uninjured, partially injured or completely injured. Peak strain, peak intervertebral extension and increases in flexibility parameters were compared among the three injury classification groups. Peak ALL strains were largest in the lower cervical spine, and increased with impact acceleration, reaching a maximum of 29.3% at C6-C7 at 8 g. Significant increases (P<0.05) over the physiological strain limits first occurred at C4-C5 during the 3.5 g trauma and spread to lower intervertebral levels as impact severity increased. The complete ligament injuries were associated with greater increases in ALL strain, intervertebral extension, and flexibility parameters than were observed at uninjured intervertebral levels (P<0.05).