Using the single-edge notched bending (SENB) test, two fracture toughness parameters of longitudinal and transverse bovine bone specimens were evaluated:​ the critical stress intensity factor, K_c, determined from the peak load to initiate fracture, and the energy or work of fracture, W_f, the energy required to extend a crack through a notched specimen. It was found that preservation of bone in alcohol resulted in a 25–45% higher K_c value compared to control specimens stored in physiological saline;​ whereas the work of fracture, W_f, demonstrated the opposite behaviour, with the alcohol stored specimens having a 28–56% lower value than the saline control specimens. It was established that the effect of alcohol is reversible upon the bone being restored in saline. Consistent with previous studies, it was found that cracks oriented in the longitudinal direction resulted in both a significantly lower fracture toughness and lower work of fracture than those cracks directed transversely. The results are discussed in terms of the proposed deformation and fracture mechanisms known to occur in bone.