A simple method for correlative light and scanning electron microscopy of human iliac crest bone biopsies:​ qualitative observations in normal and osteoporotic subjects

Dempster, D. W.; Shane, E.; Horbert, W.; Lindsay, R.

Affiliations

¹Regional Bone Center, Helen Hayes Hospital, West Haverstraw, NY

²Department of Pathology, Columbia University, New York, NY

³Department of Medicine, Columbia University, New York, NY

Abstract

Despite the fact that the scanning electron microscope (SEM) offers a number of unique advantages for the investigation of bone structure, this instrument has seldom been applied in studies of the iliac crest bone biopsy. Here we describe a very simple method of preparing iliac crest biopsies for examination in the SEM after the routine histomorphometric analysis has been performed. With observations on biopsies from normal subjects and patients with osteoporosis, the paper illustrates the potential of the method for performing correlative light and scanning electron microscopical studies and for gaining new information on the three‐dimensional architecture of trabecular bone.

Links

DOI: 10.1002/jbmr.5650010105

PubMed: 3503529

WoS: A1986C906800003

History

Received: 1985-07-31

Accepted: 1985-10-22

Cited Works (4)

Year	Entry
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Cited By (24)

Year	Entry
1988	Parfitt AM. Bone remodeling: relationship to the amount and structure of bone, and the pathogenesis and prevention of fractures. In: Riggs BL, Melton LJ III, eds. Osteoporosis: Etiology, Diagnosis, and Management. New York, NY: Raven Press; 1988:45-93.
1987	Parfitt AM. Trabecular bone architecture in the pathogenesis and prevention of fracture. Am J Med. January 26, 1987;82(suppl 1B):68-72.
1988	Parisien MV, McMahon D, Pushparaj N, Dempster DW. Trabecular architecture in iliac crest bone biopsies: infra-individual variability in structural parameters and changes with age. Bone. 1988;9(5):289-295.
1992	Parfitt AM. Implications of architecture for the pathogenesis and prevention of vertebral fracture. Bone. 1992;13(suppl 2):S41-S47.
1995	Dempster DW, Birchman R, Xu R, Lindsay R, Shen V. Temporal changes in cancellous bone structure of rats immediately after ovariectomy. Bone. January 1995;16(1):157-161.
1997	Odgaard A. Three-dimensional methods for quantification of cancellous bone architecture. Bone. 1997;20(4):315-328.
1998	Ito M, Nakamura T, Matsumoto T, Tsurusaki K, Hayashi K. Analysis of trabecular microarchitecture of human iliac bone using microcomputed tomography in patients with hip arthrosis with or without vertebral fracture. Bone. August 1998;23(2):163-169.
2012	Goff MG, Slyfield CR, Kummari SR, Tkachenko EV, Fischer SE, Yi YH, Jekir MG, Keaveny TM, Hernandez CJ. Three-dimensional characterization of resorption cavity size and location in human vertebral trabecular bone. Bone. July 2012;51(1):28-37.
1990	Mosekilde L. Consequences of the remodelling process for vertebral trabecular bone structure: a scanning electron microscopy study (uncoupling of unloaded structures). Bone Miner. July 1990;10(1):13-35.
2000	Dempster DW. The contribution of trabecular architecture to cancellous bone quality. J Bone Miner Res. January 2000;15(1):20-23.
1996	Boyde A, Jones SJ. Scanning electron microscopy of bone: instrument, specimen, and issues. Microsc Res Tech. February 1, 1996;33(2):92-120.
1992	Riggs BL, Melton LJ III. The prevention and treatment of osteoporosis. NEJM. August 27, 1992;327(9):620-627.
1995	Manolagas SC, Jilka RL. Bone marrow, cytokines, and bone remodeling: emerging insights into the pathophysiology of osteoporosis. NEJM. February 2, 1995;332(5):305-311.
2014	Cosman F, de Beur SJ, LeBoff MS, Lewiecki EM, Tanner B, Randall S, Lindsay R. Clinician’s guide to prevention and treatment of osteoporosis. Osteoporos Int. October 2014;25(10):2359-2381.
1986	Boyde A, Maconnachie E, Reid SA, Delling G, Mundy GR. Scanning electron-microscopy in bone pathology: review of methods, potential and applications. Scan Electron Microsc. 1986;1986(4):1537-1554.
1994	Sims NA. The Pathogenesis of Post-Menopausal Osteopaenia Using the Oophorectomised Rat Model [PhD thesis]. Adelaide, Australia: The University of Adelaide; July 1994.
2009	Chan M. Mechanobiology of 3D Co-Culture Trabecular Explant Model [PhD thesis]. Columbia University; 2009.
2014	Melville KM. Estrogen Receptor Alpha in Osteoblasts Mediates Bone Mass and Bone's Response to in Vivo Loading [PhD thesis]. Ithaca, NY: Cornell University; August 2014.
2015	Goff M. The Role of Micro and Ultra-Structure in Microdamage Accumulation in Cancellous Bone [PhD thesis]. Ithaca, NY: Cornell University; August 2015.
2017	Kelly NH. Transcriptomic Analysis of Cortical Versus Cancellous Bone in Response to Mechanical Loading and Estrogen Signaling [PhD thesis]. Ithaca, NY: Cornell University; January 2017.
2014	Holub O. Biomechanics of Spinal Metastases [PhD thesis]. University of Leeds; April 2014.
2011	Muir JW. Evaluating the Potential of Low Magnitude Mechanical Signals as a Means of Preserving Postural Control in the Aged and Disabled [PhD thesis]. Stony Brook, NY: Stony Brook University; August 2011.
2004	McNamara LM. Biomechanical Origins of Osteoporosis [PhD thesis]. Trinity College Dublin; March 2004.
2008	Mulvihill BMC. Computational Investigation Into the Mechanoregulation of Osteoporosis [PhD thesis]. Trinity College Dublin; December 2008.