This paper presents a multivariate analysis on the impact of the exclusive motorcycle lane on motorcycle accidents along the Federal Highway Route 2, Malaysia. A number of statistical models have been developed to explain the relationship of motorcycle accidents and explanatory variables relevant to motorcycle safety. The best model showed that motorcycle accidents are directly proportional to the cubic power of traffic flow and reduced by approximately 39% with the motorcycle lane. A clear benefit of this lane is observed when the traffic flow exceeds 15,000 vehicles per day per lane for motorcycle proportion of between 20% and 30%. Besides supporting the notion for motorcycle segregation, this finding provides an initial guideline on the warrants for an exclusive motorcycle lane for highly motorcycled countries in Asia.