A study of trabecular bones in ovariectomized goats with micro-computed tomography and peripheral quantitative computed tomography

Siu, W. S.; Qin, L.; Cheung, W. H.; Leung, K. S.

Affiliations

¹Department of Orthopaedics and Traumatology, The Chinese University of Hong Kong, China

Abstract and keywords

Osteoporotic fractures occur most frequently in trabeculae-rich skeletal sites. The purpose of this study was to use a high-resolution micro-computed tomography (micro-CT) to investigate the changes in trabecular bone microarchitecture and to use a peripheral quantitative computed tomography (pQCT) to study changes in volumetric bone mineral density (BMD) in a large animal model resulted from ovariectomy (OVX). Ten adult goats were used for this study. The first iliac crest biopsy was harvested before OVX and served as baseline; the second biopsy was collected 6 months later from the opposite side for both pQCT and micro-CT measurements. Results showed that after 6 months of OVX, the BMD of the iliac crest biopsies decreased significantly by 16.3% (P < 0.05). The bone volume density (BV/TV), trabecular number (Tb.N), and connectivity density (Conn.D) measured with micro-CT decrease significantly after OVX, with an average decrease of 8.34%, 8.51%, and 18.52% (P < 0.05 each), respectively. The trabecular plate separation (Tb.Sp) was 8.26% (P < 0.05) greater than baseline after OVX. Significant correlations were found between the reduction of BMD and the decreases of BV/TV and Tb.N (r = 0.839 and 0.719, respectively; P< 0.001 both), as well as the increase of Tb.Sp (r = −0.758, P< 0.001) and SMI (r = −0.697, P< 0.001). In conclusion, this was the first experimental study in goat model to show that OVX-induced bone loss in goats was attributed by deterioration of trabecular microarchitecture.

Keywords: Micro-CT; pQCT; Trabecular bone; Osteoporosis; Microarchitecture

Links

DOI: 10.1016/j.bone.2004.03.014

PubMed: 15207737

WoS: 000222494100004

History

Received: 2003-05-12

Revised: 2004-03-11

Accepted: 2004-03-16

Online: 2004-06-17

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Cited By (6)

Year	Entry
2005	Gong H, Zhang M, Yeung HY, Qin L. Regional variations in microstructural properties of vertebral trabeculae with aging. J Bone Min Metab. March 2005;23(2):174-180.
2011	Yan Y-B, Qi W, Wang J, Liu L-F, Teo E-C, Tianxia Q, Ba J-j, Lei W. Relationship between architectural parameters and sample volume of human cancellous bone in micro-CT scanning. Med Eng Phys. July 2011;33(6):764-769.
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2014	Fonseca H, Moreira-Gonçalves D, Coriolano H-JA, Duarte JA. Bone quality: the determinants of bone strength and fragility. Sports Med. January 2014;44(1):37-53.
2021	Fraschetti A. The Effects of Exposure to Trace Elements on the Skeletal Health of American Mink [Master's thesis]. Hamilton, ON: McMaster University; December 2021.
2017	Kreipke TC. Structural, Mechanical, and Biological Relationships of Trabecular Bone in Osteoporosis [PhD thesis]. Notre Dame, IN: University of Notre Dame; April 2017.