Ptychographic X-ray CT characterization of the osteocyte lacuno-canalicular network in a male rat's glucocorticoid induced osteoporosis model

Ciani, Antonia; Toumi, Hechmi; Pallu, Stéphane; Tsai, Esther H.R.; Diaz, Ana; Guizar-Sicairos, Manuel; Holler, Mirko; Lespessailles, Eric; Kewish, Cameron M.

Affiliations

¹Synchrotron Soleil, L'Orme des Merisiers, 91192 Gif-sur-Yvette, France

²EA4708, Imagerie Multimodale, Multiéchelles et Modélisation du Tissu Osseux et Articulaire (I3MTO), Université d'Orléans, 45000 Orléans, France

³Département Rhumatologie, Centre Hospitalier Régional d'Orléans, 45067 Orléans, France

⁴Paul Scherrer Institut, 5232 Villigen, Switzerland

⁵Current address: UMR CNRS 7052 CNRS Laboratoire de Bioingénierie et Bioimagerie Ostéoarticulaire (B2OA), UFR de Médecine, Université Paris Diderot, 75010 Paris, France

⁶Current address: ARC Centre of Excellence in Advanced Molecular Imaging, La Trobe University, Bundoora VIC 3086, Australia; and Australian National Science and Technology Organisation, Australian Synchrotron, Clayton VIC 3168, Australia

Abstract and keywords

Ptychographic X-ray computed tomography (PXCT) is a quantitative imaging modality that non-destructively maps the 3D electron density inside an object with tens of nanometers spatial resolution. This method provides unique access to the morphology and structure of the osteocyte lacuno-canalicular network (LCN) and nanoscale density of the tissue in the vicinity of an osteocyte lacuna. Herein, we applied PXCT to characterize the lacunae and LCN in a male Wistar rat model of glucocorticoid-induced osteoporosis (GIO). The ptychographic images revealed significant (p < 0.05) differences in the number of canaliculi originating from the lacuna per ellipsoidal surface unit, Ca.Nb (p = 0.0106), and the 3D morphology of the lacuna (p = 0.0064), between GIO and SHAM groups. Moreover, the mean canalicular diameter, Ca.Dm, was slightly statistically un-significantly smaller in GIO (152 ± 6.5) nm than in SHAM group (165 ± 8) nm (p = 0.053). Our findings indicate that PXCT can non-destructively provide detailed, nanoscale information on the 3D organization of the LCN in correlative studies of pathologies, such as osteoporosis, leading to improved diagnosis and therapy.

Keywords: Osteocytes; Glucocorticoid-induced osteoporosis; Nano-CT; Quantitative analysis; Ptychography

Links

DOI: 10.1016/j.bonr.2018.07.005

PubMed: 30246062

PubMedCentral: PMC6146379

History

Received: 2018-05-21

Accepted: 2018-07-27

Online: 2018-07-29

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