Characterization of a bovine synovial fluid lubricating factor, I: chemical, surface activity and lubricating properties

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Jay, Gregory D.¹

Characterization of a bovine synovial fluid lubricating factor, I: chemical, surface activity and lubricating properties

Connect Tissue Res. 1992;28(1-2):71-88

©1992 Gordon and Breach Science Publishers S.A.

Affiliations

¹Department of Pathology, State University of New York at Stony Brook, Stony Brook, New York, USA
Abstract and keywords

A lubricating glycoprotein (PSLF) with an apparent molecular weight of 280 kDa was purified from bovine synovial fluid by anion exchange, molecular sieve chromatography, and density gradient centrifugation. Lubrication was measured under boundary conditions as lowering of the coefficient of friction (μ) between oscillating natural latex and polished glass. Lubricating ability was first observed at a concentration of 200 μg/ml and became maximal at 260 μg/ml. Hydrophobic interfacial tension measurements indicated that at the former concentration, monolayers of PSLF formed. Sugar digestions showed that lubricating ability depends upon the terminal galactose of the molecule. PSLF is similar if not identical to lubricin. It is proposed that a repulsive hydration force is the molecular mechanism for lubricating activity.

Keywords: Tribology; cartilage; synovial fluid; lubricin; repulsive hydration force
Links

DOI: 10.3109/03008209209014228

PubMed: 1628491

WoS: A1992HV38600005
History

Received: 1991-02-19

Revised: 1991-08-29

Accepted: 1991-12-17

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Cited By (31)

Year	Entry
2007	Schmidt TA, Gastelum NS, Nguyen QT, Schumacher BL, Sah RL. Boundary lubrication of articular cartilage: role of synovial fluid constituents. Arthritis Rheum. March 2007;56(3):882-891.
2007	Jay GD, Torres JR, Rhee DK, Helminen HJ, Hytinnen MM, Cha C-J, Elsaid K, Kim K-S, Cui Y, Warman ML. Association between friction and wear in diarthrodial joints lacking lubricin. Arthritis Rheum. November 2007;56(11):3662-3669.
2008	Elsaid KA, Fleming BC, Oksendahl HL, Machan JT, Fadale PD, Hulstyn MJ, Shalvoy R, Jay GD. Decreased lubricin concentrations and markers of joint inflammation in synovial fluids from patients with anterior cruciate ligament injury. Arthritis Rheum. June 2008;58(6):1707-1715.
2008	Gleghorn JP, Bonassar LJ. Lubrication mode analysis of articular cartilage using Stribeck surfaces. J Biomech. 2008;41(9):1910-1918.
2009	Ateshian GA. The role of interstitial fluid pressurization in articular cartilage lubrication. J Biomech. June 19, 2009;42(9):1163-1176.
2023	Pellicore MJ, Gangi LR, Murphy LA, Lee AJ, Jacobsen T, Kenawy HM, Shah RP, Chahine NO, Ateshian GA, Hung CT. Toward defining the role of the synovium in mitigating normal articular cartilage wear and tear. J Biomech. February 2023;148:111472.
2023	Dogru S, Dai Z, Alba GM, Simone NJ, Albro MB. Computational and experimental characterizations of the spatiotemporal activity and functional role of TGF-β in the synovial joint. J Biomech. July 2023;156:111673.
1997	Ateshian GA. A theoretical formulation for boundary friction in articular cartilage. J Biomech Eng. February 1997;119(1):81-86.
2001	Jay GD, Tantravahi U, Britt DE, Barrach HJ, Cha C-J. Homology of lubricin and superficial zone protein (SZP): products of megakaryocyte stimulating factor (MSF) gene expression by human synovial fibroblasts and articular chondrocytes localized to chromosome 1q25. J Orthop Res. July 2001;19(4):677-687.
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