Bone biomineral properties vary across human osteonal bone

Wittig, Nina K.; Palle, Jonas; Østergaard, Maja; Frølich, Simon; Birkbak, Mie E.; Spiers, Kathryn M.; Garrevoet, Jan; Birkedal, Henrik

Affiliations

¹Department of Chemistry and iNANO, Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark

²DESY Photon Science, Notkestr. 85, D-22607 Hamburg, Germany

Abstract and keywords

The biomineralization of bone remains a puzzle. During Haversian remodeling in the dense human cortical bone, osteoclasts excavate a tunnel that is then filled in by osteoblasts with layers of bone of varying fibril orientations, resulting in a lamellar motif. Such bone represents an excellent possibility to increase our understanding of bone as a material as well as bone biomineralization by studying spatio/temporal variations in the biomineral across an osteon. To this end, fluorescence computed tomography and diffraction scattering computed tomography with sub-micrometer resolution is applied to obtain position resolved fluorescence spectra and diffraction patterns in a 3D volume. The microstructural properties of the apatite biomineral are not homogeneous but depend critically on the time point at which it was laid down. This indicates that the nature of bone biomineral is highly dependent on the microenvironment during bone formation and remodeling.

Keywords: biomineralization; hierarchical materials; diffraction scattering computed tomography; fluorescence computed tomography; bone

Links

DOI: 10.1021/acsnano.9b05535

History

Received: 2019-07-15

Accepted: 2019-10-15

Online: 2019-10-15

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Cited By (5)

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2021	Raguin E, Rechav K, Shahar R, Weiner S. Focused ion beam-SEM 3D analysis of mineralized osteonal bone: lamellae and cement sheath structures. Acta Biomater. February 2021;121:497-513.
2023	Østergaard M, Naver EB, Schüpbach D, Kaestner A, Strobl M, Brüel A, Thomsen JS, Schmidt S, Poulsen HF, Kuhn LT, Birkedal H. Correlative study of liquid in human bone by 3D neutron microscopy and lab-based X-ray μCT. Bone. October 2023;175:116837.
2023	Grünewald TA, Johannes A, Wittig NK, Palle J, Rack A, Burghammer M, Birkedal H. Bone mineral properties and 3D orientation of human lamellar bone around cement lines and the Haversian system. IUCrJ. October 2023;10(2):189-198.
2023	Tang T, Landis W, Blouin S, Bertinetti L, Hartmann MA, Berzlanovich A, Weinkamer R, Wagermaier W, Fratzl P. Subcanalicular nanochannel volume is inversely correlated with calcium content in human cortical bone. J Bone Miner Res. February 2023;38(2):313-325.
2022	Wittig NK, Østergaard M, Palle J, Christensen TEK, Langdahl BL, Rejnmark L, Hauge E-M, Brüel A, Thomsen JS, Birkedal H. Opportunities for biomineralization research using multiscale computed X-ray tomography as exemplified by bone imaging. J Struct Biol. March 2022;214(1):107822.