The ultrastructure of bone as revealed in electron microscopy of ion-milled sections

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Schwarcz, Henry P.¹

The ultrastructure of bone as revealed in electron microscopy of ion-milled sections

Semin Cell Dev Biol. October 2015;46:44-50

©2015 Elsevier Ltd. All rights reserved.

Affiliations

¹School of Geography and Earth Sciences, McMaster University, Hamilton, ON, Canada
Abstract

Mineral makes up more than half the volume of bone, but its spatial and structural relationship to collagen and other proteins is still a matter of debate. Due to the nanometer-size of bone crystals this matter can be resolved only with transmission electron microscope (TEM) images. Using sections cut with an ultramicrotome, previous investigators determined most mineral lies in the 40 nm wide gap zone in collagen fibrils. Using less invasive sectioning methods (ion milling and focused ion beam [FIB]) reveals that most mineral is extrafibrillar, occurring in the form of mineral lamellae, polycrystalline plates 300 nm or more long, packed around collagen fibrils in stacks of four or more lamellae <1 nm apart. While Ca and P also occur in the gap zone, they do not appear to be in the form of well-crystallized apatite. This new model for bone ultrastructure resolves outstanding problems presented by the previous model.
Links

DOI: 10.1016/j.semcdb.2015.06.008

PubMed: 26165821

WoS: 000366137400007
History

Received: 2015-03-4

Revised: 2015-05-22

Accepted: 2015-06-28

Online: 2015-07-9

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Cited By (13)

Year	Entry
2020	Schwarcz HP, Binkley DM, Luo L, Grandfield K. A search for apatite crystals in the gap zone of collagen fibrils in bone using dark-field illumination. Bone. June 2020;135:115304.
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2020	Binkley DM, Deering J, Yuan H, Gourrier A, Grandfield K. Ellipsoidal mesoscale mineralization pattern in human cortical bone revealed in 3D by plasma focused ion beam serial sectioning. J Struct Biol. November 1, 2020;212(2):107615.
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2023	Micheletti C. Multimodal and Multiscale Characterization of Bone and Bone Interfaces in Health and Disease [PhD thesis]. University of Gothenburg; 2023.
2023	Buss DJ. Mineralization of Biological Fiber Systems [PhD thesis]. McGill University; July 2023.
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2019	Lee BEJ. Characterization Strategies for Bone Ultrastructure and Bone-Cell Interfacing Materials [PhD thesis]. Hamilton, ON: McMaster University; November 2019.
2019	Strakhov I. The Nanoscale Structure of Human Female Osteoporotic Bone Investigated by Transmission Electron Microscopy [Master's thesis]. Hamilton, ON: McMaster University; September 2019.