Musculoskeletal simulations of human movement commonly use Hill muscle models to predict muscle forces, but their sensitivity to model parameter values is not well understood. The purpose of this study was to evaluate muscle model sensitivity to perturbations in 14 Hill muscle model parameters in forward dynamic simulations of running and walking by varying each by ±50%. Three evaluations of the muscle model were performed based on:​ (1) calculating the sensitivity of the muscle model only, (2) determining the continuous partial derivatives of the muscle equations with respect to each parameter, and (3) evaluating the effects on the running and walking simulations. Model evaluations were found to be very sensitive (percent change in outputs greater than parameter perturbation) to parameters defining the series elastic component (tendon), force–length curve of the contractile element and maximum isometric force. For some parameters, the range of literature values was larger than the model sensitivity. Model evaluations were insensitive to parameters defining the parallel elastic element, force–velocity curve of the contractile element and muscle activation time constants. The derivative method provided similar results, but also provided a generic, continuous equation that can easily be applied to other motions. The sensitivities of the running and walking simulations were reduced compared to the sensitivity of the muscle model alone. Results demonstrate the importance of evaluating sensitivity of a musculoskeletal simulation in a controlled manner and provide an indication of which parameters must be selected most carefully based on the sensitivity of a given movement.